摘要
目的探讨RET基因的表达情况与先天性巨结肠发生的关系。方法采用RTPCR技术对12例先天性巨结肠的狭窄段、移行段及扩张段分别做RET基因的表达,并以G3PDH作内参标,观察RET基因的表达情况。结果发现RET基因的表达从扩张段→移行段→狭窄段是逐渐减低的。
Objective To investigate the relationship between the expression of RET proto oncogene and the pathogenesis of Hirschsprung's disease. Methods The expression of RET proto oncogene in the aganglionic, transitional and dilated segments of colon from 12 children with Hirschsprung's disease was studied by RT PCR method. G3PDH was adopted as control.Results The expression of RET proto oncogene from the dilated segment to the transitional segment and aganglionic segment decreased gradually.Conclusions Expression of RET proto oncogene plays an important role in the pathogenesis of Hirschsprung's disease.
出处
《中华小儿外科杂志》
CSCD
1999年第1期5-6,共2页
Chinese Journal of Pediatric Surgery
关键词
先天性
巨结肠症
基因表达
聚合酶链反应
Hirschsprung's disease Gene expression Polymerase chain reaction