新生猪仔肠缺血再灌注所致细菌移位的实验研究

Bacterial Translocation in Mesenteric Ischemia reperfusion Injury in Newborn Piglets

目的了解新生儿肠缺血再灌注损伤对细菌移位的影响。方法采用新生猪仔非阻塞性肠缺血再灌注模型，观察不同程度缺血再灌注所致肠道细菌移位及肠粘膜上皮损伤情况。结果对照组无细菌移位；中度缺血仅有肠系膜淋巴结细菌移位；中度缺血再灌注、重度缺血、重度缺血再灌注均导致肠系膜淋巴结、外周血、肝、脾细菌移位。缺血再灌注组细菌移位数明显高于仅有缺血组（Ｐ＜０．０１），重度缺血再灌注可见肠粘膜上皮损伤。结论新生猪仔肠缺血再灌注可引起细菌移位及肠粘膜损伤，其严重程度与缺血严重程度及有无再灌注有关。这一病理改变应引起临床重视。

Objective To study bacterial translocation during mesenteric ischemia reperfusion injury.Methods Bacterial translocation (BT) and intestinal mucosal damage were studied in newborn piglets subjected to various degress of non occlusive ischemia reperfusion injury.Results The control group (group Ⅰ) did not reveal any BT. 83% of piglets subjected to moderate degree of ischemia (group Ⅱ) had BT to mesenteric lymph nodes. Piglets subjected to moderate ischemia reperfusion (group Ⅲ), severe ischemia alone (group Ⅳ) and severe ischemia reperfusion (group Ⅴ) did not only grow positive culture in mesenteric lymph nodes, but also in peripheral blood, liver and spleen. The incidence of BT was higher in ischemia perfusion groups than in the groups with ischemia alone ( P < 0.01 ). Intestinal mucosal damage was detected histologically in the severe ischemia perfusion group.Conclusions Ischemia reperfusion injury induced BT and intestinal mucosal damage in newborn piglets. The extent of BT and the presence of mucosal damage correlate with the severity of ischemia reperfusion injury.