递增负荷运动至力竭大鼠肾脏自由基产生及氧化抗氧化能力的研究

A Study on Production of Free Radical and Oxidative,Anti oxidative Capability of kidney in Rats During and After Incremental Exercise to Exhaustion

以大鼠增负荷力竭性运动为模型，利用低温电子自旋共振（ＳＥＲ）技术，分别于安静时、运动中、运动后即刻、运动后３０ｍｉｎ、２ｈ、４ｈ、８ｈ提取大鼠肾组织，测定氧自由基（ＯＦＲ）信号强度，同时测定ＳＯＤ活力和ＭＤＡ含量，结果表明：①运动后恢复期３０ｍｉｎ，肾脏ＯＦＲ信号强度升高明显，提示运动性肾缺血及恢复期再灌注诱导的黄嘌呤氧化酶机制可能是肾组织ＯＦＲ生成的主要来源；②在运动过程中及恢复期ＯＦＲ改变与ＳＯＤ变化趋势基本吻合，提示肾内源性ＳＯＤ在防止ＯＦＲ损伤中起重要作用；③肾脏ＭＤＡ于运动后２ｈ出现峰值，而ＯＦＲ则于恢复期３０ｍｉｎ升至最高，说明除肾脏自身产生活性氧诱导脂质过氧化以外。

Taking incremental exercise to exhaustion as the experimental model and using low temperature electron spin resonance(ESR) technique as the determining method,we sampled rats kidney tissue at rest,druing exercise and post exercise at 0,30,120,240 and 480 min., respectively.the signal strength of OFR species,SOD activity and MDA content were measured.The results showed that:1.The signal strength of OFR species increased apparently at 30 min. post exercise. It indicated that ischemia_reperfusion mechanism might be the major resource of OFR.2.The changes of OFR species coincided with SOD during exercise and recovery,which suggested that SOD might play an important role in protecting kidney from OFR damage.3.MDA content increased to the highest level at 2h post exercise,while the signal strength of OFR reached peak level at 30 min.post exercise,which showed that MDA transferring from other organs might be of a large portion besides kidney produced OFR and induced lipid peroxidation itself.