摘要
球形棕囊藻生活史中包含游离单细胞和球形囊体两种生活形态,但是实验室中培养的球形棕囊藻经常无法形成囊体。研究通过向培养基中泵入过滤空气,以及给培养基提供不同程度的搅动,研究了充气和搅动对球形棕囊藻生长及囊体形成的影响。充气和搅动均显著提高了囊体的数量,并且提高了囊体内细胞的生长速率。但是充气对于囊体直径及囊体内细胞密度并无显著影响。搅动则明显的提高了囊体直径和囊体内细胞数量。然而,尽管充气以及搅动有利于球形棕囊藻囊体的形成,但是培养的囊体直径依然小于自然海区中囊体的大小。
Phaeocystis globosa scherffel has a polymorphic life cycle alternating between free-living cells and colonies.Phaeocystis usually lacks the abilities to form the colonies under lab-controlled conditions.In this work,we investigated the effects of air bubbling and turbulence on the growth and colony-forming of P.globosa.Both air bubbling and turbulence favored P.globosa to form more colonies.However,only turbulence greatly enhanced the colony size and colonial cell numbers,whereas air bubbling did not exhibit significant impacts.Nonetheless,the colonies developed in the lab still had lower diameters than the natural ones.
出处
《生态学报》
CAS
CSCD
北大核心
2010年第12期3368-3374,共7页
Acta Ecologica Sinica
基金
国家自然科学基金资助项目(30670156)
国家自然科学基金-广东省联合基金重点资助项目(U0733006)
高等学校博士学科点专项科研基金资助项目(20070574003)
973国家重点基础研究发展计划资助项目(2010CB428702)
国家科技部863资助项目(2006AA09Z178)
关键词
球形棕囊藻
囊体形成
充气
搅动
Phaeocystis globosa
colony-forming
air bubbling
turbulence
