次声作用引起大鼠下丘脑c-fos mRNA与CRF mRNA的表达

The expression of c-fos and CRF mRNA in hhpothalamus of rats after infrasound exposure

目的：了解次声引起应激反应的中枢机制．方法：采用反转录ＰＣＲ方法，研究了８Ｈｚ，１２０ｄＢ次声作用后不同时间引起大鼠下丘脑ｃ－ｆｏｓｍＲＮＡ及促肾上腺皮质激素释放因子（ＣＲＦ）ｍＲＮＡ的表达情况．结果：８Ｈｚ，１２０ｄＢ次声作用２ｈ，于作用后０．５ｈｃ－ｆｏｓ与ＣＲＦｍＲＮＡ均开始明显表达，随时间延长，表达渐增强，ｃ－ｆｏｓ于２ｈ达高峰，至４ｈ基本消失；ＣＲＦ于３ｈ达高峰，至５ｈ基本消失．结论：次声作用后ｃ－ｆｏｓ与ＣＲＦｍＲＮＡ的表达可能是次声引起应激反应的中枢分子机制．

AIM: To explore the central nerves mechanism ofinfrasound induced stress. METHODS: The expression of cfos and cortiootropin--releasing factor (CRP ) mRN A in hypothalamus after infrasound exposure of rats had been investigated by reverse transcription polyserase chain reaction(RT--PCR) method. RESULTS: Rats exposed to 8 Hz, 120dB infrasound for 2 h, after 0. 5 h the c-fos mRNA and CRFmRNA were significantly expressed in the hypothalamus ofrats. The c--fos mRNA expression disappeared in 4 h after infrasound exposure; otherwise, the CRF mRNA expressiondisappeared in 5 h. CONCLUSION: The result suggestedthat c--fos and CRF take some important role in infrasound induced stress.