血管生成及细胞凋亡与喉粘膜上皮癌变的关系

Relationship of apoptosis and angiogenesis and the carcinogenesis of laryngeal mucosa

目的：探讨血管生成及细胞凋亡与喉粘膜上皮癌变的关系．方法：应用免疫组化和原位末端标记（ＩＳＥＬ）方法分别检测正常喉粘膜（Ｎ）、炎症（ＩＦ）、不典型增生（ＤＹＳ）和鳞癌（ＳＣＣ）中间质的微血管数量及上皮的细胞凋亡情况，标记后的血管和凋亡细胞通过计数分别以微血管密度（ＭＶＤ）及凋亡指数（ＡＩ）表示．结果：ＭＶＤ值从Ｎ，ＩＦ，ＤＹＳ至ＳＣＣ依次增高，ＤＹＳ及ＳＣＣ组的ＭＶＤ值与Ｎ，ＩＦ组存在显著性差异（Ｐ＜０．０５）；ＤＹＳ组的凋亡指数（ＡＩ）在所有病变中最高；ＤＹＳ及ＳＣＣ组的ＡＩ值较正常Ｎ及ＩＦ组明显增多，且有显著性差异（Ｐ＜０．０５）．结论：血管生成及细胞凋亡与喉粘膜癌前病变和癌变关系密切，在喉粘膜上皮癌变发生中起着重要的作用．

AIM: To determine the relationship of apoptosisand angiogenesis with the carcinogenesis of laryngeal mucosa.METHODS: The distribution patterns of apoptosis and angiogenesis in normal (N), chronic inflammation (IF), dysplasia(DYS ) and squamous cell carcinoma (SCC ) of laryngealmucosa were observed quantitatively by immunohistochemistry and in situ end--labeling methods. The apoptosisindex (AI ) represented the percentage of positive labeledapoptotic cells in all measured cells, and the micro--vessel density (MVD) represented the number of counted vessels in thestroma. RESULTS: The MVDs of SCC and DYS were significantly higher than that of N and IF groups (P<0. 05). AI ofDYS was the highest one in all observed groups. The AIs ofDYS and SCC were significantly higher than that of N and IF(P<0. 05 ). CONCLUSION: The angiogenesis and apoptosiswere highly associated with the precancerosis and carcinogenesis of laryngeal mucosa, They play important roles in thecarcinogenesis of laryngeal mucosa.