间隙连接蛋白Cx32在肝细胞癌组织中的表达

Expression of gap junction protein connexin 32 in human hepatocellular carcinoma,pericancerous liver and normal liver tissues

目的：探讨间隙连接蛋白Ｃｘ３２在肝细胞癌（ｈｅｐａｔｏ－ｃｅｌｌｕｌａｒｃａｒｃｉｎｏｍａ，ＨＣＣ）、癌旁肝及正常肝组织中的表达和意义．方法：应用ＳＰ免疫组织化学法检测Ｃｘ３２在４７例ＨＣＣ，１５例癌旁肝及９例正常肝组织中的表达．结果：Ｃｘ３２蛋白表达既定位于肝细胞胞质中，又定位于胞膜上．Ｃｘ３２在ＨＣＣ、癌旁肝及正常肝组织中的阳性率分别为４１．７％，７３．３％及８８．９％．经ｘ２检验，Ｃｘ３２在ＨＣＣ中的表达明显低于在癌旁肝及正常肝组织的水平（Ｐ＜０．０１）．随着ＨＣＣ分化程度的降低，其阳性率有所下降（Ｐ＜０．０５）．结论：间隙连接蛋白Ｃｘ３２表达减弱可能与ＨＣＣ的发生密切相关．

AIM: To investigate the regularity and significanceof expression of connexin 32 (Cx32) in human hepatocellularcarcinoma (HCC), pericancerous liver and normal liver tissues. METHODS: Expression of Cx32 in 47 cases of humanHCC, 15 cases of pericancerous liver tissues and 9 oasses ofnormal heptocyres were detected by SP immunohistochemicalmethod. RESULTS: Cx32 antigen postive stain located notonly in the eytopiasm but also on hte membranes of hepatceytes. The dotoetion rates of Cx32 in HCC, poricancerousliver tissue and notmal liver tissues were 41. 7%, 73. 3% and8 8. 9% respectively. In HCC degree, the rates are50. 0%, 42. 9% and 38. 9%. The lower degree, the lowerpositive rate. Chi-square test suggested that Cx32 positiverate in HCC was significant lower than that in pericancerousliver tissur and normal liver tissues (P< 0.01). CONCLUSION: The decrease of Cx32 protein might play an importantrole in hepatocarcinogenesis.