卵巢恶性肿瘤p53基因5-7外显子突变及蛋白阳性的生物学意义

Study on the relationship between protein overexpression and mutations of e-cons5-7 in p53 and clinical biological behavior of malignant ovarian tumors

目的：比较研究卵巢恶性肿瘤ｐ５３基因第５，６，７外显子突变与ｐ５３基因蛋白的过度表达和临床生物学行为的相关性．方法：对５０例卵巢恶性肿瘤及１０例良性肿瘤石蜡切片进行p５３蛋白免疫组化检测，其中２３例卵巢恶性肿瘤的新鲜标本进行了ｐ５３基因第５，６，７外显子ＰＣＲ－ＳＳＣＰ检测．结果：５０例卵巢恶性肿瘤中１２例Ｐ５３蛋白阳性表达２４％（１２／５０）．上皮癌２９％（９／２８）生殖细胞肿瘤１０％（１／１０）间质细胞瘤２５％（２／８），转移瘤２５％（１／４）在２３例标本ＰＣＲ－ＳＳＣＰ检测中突变率为３５％（８／２３）第５，６．７外显子突变率分别为９％（２／２３），９％（２／２３）．１７％（４／２３）．两者均阳性者为２例．即ＰＣＲ－ＳＳＣＰ检测和蛋白表达均阳性第６．７外显子各１例．结论：①卵巢恶性肿瘤存在ｐ５３基因突变蛋白的过度表达．但突变与蛋白阳性表达并非完全一致；

AIM: To study the telatlonship between the muta-tions of exons 5-7 in p53 gene and protein overexpression and clinical biological behavior of malignant ovarian tumors METHJODS:Immunohistochemical staining method was used to examine the Parafin embeuueu sections of 50 cases of malignant ovarian and tumors and 10 cases of benign tumors.Analysis of e-cons 5--7 of the p53 gene was performed in 23 o-varian malignance. RESULTS: Positive expression rate of p53for ovarian maligllancc was 24 % (12/50), epithelial cell type29%(8/28 ), genetic cell type 10% (1/10 ), interstitial celltype 25% (2/8 ), and metastatic tumors 25% (1/4 ), Amongthe 23 ovarian malignance subjected to PCR--SSCP test, therewere 35% (8/23), 9% (2/23) in exon 5, 9% (2/23) in exon6; 17% (4/23) in cxon 7. Two were posillve for both PCR-SSCP and immunostaining test. One was in exon 6, anotherwas in exon 7. CONCLUSION: Mutations of the p53 tu-mor suppressor gene have overexpression of p53 protein in o-varian malignance. but they do not coincide; Mutation ofthe p53 gene and overexprcssion of P53 protein have no obvi-ous reiations with pathological types and clinical stages.