摘要
目的:建立阿维莫泮胶囊的高效液相色谱(HPLC)含量测定方法。方法:液相分离采用SHIMADZUVPODS分析柱,柱温:30℃,以甲醇-0.05mol.L-1KH2PO4(pH2.8)溶液洗脱,流速为1.0ml.min-1,进样量20μl;检测波长:254nm;样品用流动相超声溶解,过滤后进样分析。结果:辅料及中间体均不干扰样品的测定;在5.0～1000mg·mL-1范围内阿维莫泮呈良好的线性关系,相关系数r=0.9999;回收率(n=9)为99.6%;日内和日间RSD均<1.0。结论:本方法操作简便、选择性好、准确、灵敏,可用于阿维莫泮的含量测定。
Objective：To develop a validated, highly sensitive and selective high performance liquid chromatographic （HPLC） method for the quantitative determination of alvimopan. Methods：After dissolve with mobile phase,20 p.l of sample solution was injected onto a Hypeersil ODS column and the effluont was measured for UV absorption at 254nm. A mixture of 0.01mol· L - 1 NaH2 po4 （ pH 3.0 ） - acetonitrile （75：25 ） was used as the mobile phase with the flow rate of 1.0mL min -1. Results ：The linear concentration range for alvimopan was 5.0 - 1000mol · L - 1 （ r = 0. 9999 ）, extraction recovery was 99.6% - 100.3% for alvimopan. The RSDs of intra - and inter - day were less than 1.0%. Conclusion：The method is sensitive and repeatable enough to be used in assay studies.
出处
《医学信息（中旬刊）》
2010年第7期1670-1671,共2页
Medical Information Operations Sciences Fascicule
