玉米秸秆水解液发酵联产氢气与2,3-丁二醇的初步研究

Simultaneous fermentation of hydrogen and 2,3-butanediol using hydrolysate of corn straw

选用实验室自行筛选的Klebsiella pneumoniae ECU-15,进行了玉米秸秆水解液发酵联产氢气和2,3-丁二醇的初步研究。结果表明:以葡萄糖为碳源时,两目标产物随培养条件的改变呈现相同的变化趋势,且最佳发酵温度为37℃,最佳pH为6.0,最佳初始糖浓度为30 g/L;不同比例葡萄糖/木糖为混合碳源时,均能实现氢气和2,3-丁二醇的联产过程,但随着木糖含量的增加,细胞产量、氢气产量和2,3-丁二醇的产量都有所下降,并且木糖的存在会降低葡萄糖的消耗速率;实验最后以玉米秸秆水解液和同比例模拟合成培养基为底物,初步探明了该菌株利用水解液发酵联产氢气和2,3-丁二醇的可行性,最终氢气产量为0.65 v/v,产氢得率为0.43 mol/mol sugar;2,3-丁二醇产量为5.05 g/L,得率为0.82 mol/mol sugar。

Klebsiella pneumoniae ECU-15 was examined for its ability to produce hydrogen and 2,3-butanediol from hydrolysate of corn straw. Effects of culture conditions on the simultaneous fermentation indicated that the objective products showed the same change trend as the variation of the culture condition, and the optimum temperature, pH and initial glucose were 37＊（2, 6.0 and 30g/L, respectively. Batch fermentation of the xylose and glucose mixed in different ratios demonstrated that the simultaneous fermentation could be carried out, and the production of cell, hydrogen and 2, 3-butanediol were all decreased with the increasing of xylose in the substrate. The result also indicated that the utilization rateof glucose reduced due to the present of xylose. In the end, simultaneous fermentation of hydrogen and 2, 3- butanediol using hydrolysate was preliminarily studied. The production of hydrogen and 2,3-butanediol was 0.65 v/v and 5.05 g/L; the yield of them was 0.43 mol/tool sugar and 0.82 mol/tool sugar, respectively.