摘要
该文报道了碳源、氮源及吸附剂对香荚兰细胞悬浮培养产生香兰素的影响，结果表明，蔗糖比葡萄糖及果糖更适合作香荚兰细胞生长及产生香兰素的碳源，最佳蔗糖浓度为５％；当培养基中仅含ＫＮＯ３，则有利于细胞的生长和香兰素的形成，培养液中去掉ＫＮＯ３仅含ＮＨ４ＮＯ３时，细胞生长和香兰素形成均被抑制；培养基添加吸附剂后，香美兰细胞产生的香兰素含量明显增加，活性炭的效果优于ＸＡＤ-２，而且活性炭用量增加，香兰素的产量亦增加。
This article reported the effects of the kinds and concentration of carbon sources.nitrogen sources and absorbents on the cell growth and producation of vaniliin of Yanillaplanifonia cells in batch suspension culture. It was shown that sucrose was the better carbonsource than glucose and fructose foe the cell growth and vanillin formation: and when itsconcentrion in MS medium was of 5 percentage. the biosynthesis of vanillin had thelargestnumerical value: nitrate -- nitrogen improved cell growt}l and vanillin producation. butammonia-nitrogen inhibited vanillin formation;;;; the usage of absorbents (e. g. XAD - 2.activated charcoal) had a marked increasing effect on the accumulation of vanillin.
出处
《生物技术》
CAS
CSCD
1999年第1期8-10,32,共4页
Biotechnology
关键词
香美兰
香兰素
悬浮培养
Vallisa planifonia
vanillin
suspension culture
