摘要
乳猪心房组织提取液经分子膜截留,去除大分子,小分子部分经SePhPdexG-25凝胶分离,SP离子交换层析,RP-HPLCC18柱进一步纯化,获得了均一的心钠素,活性鉴定表明它有较高的利尿、利钠和降压活性。
The extraction of artrial tissue of neonatal porcine was ultrafiltered to remove largermolecular;; the lower molecular (Mw<10 000Da) was collected for isolation by sephadex G-25 gel;; purification by SP ion - exchange and further purification by RP - HPLC. Theproduct was tested by IEF;; showed that purified cardionatrin was acquired. It also showedhigh active of dilution;; natriuretic response;; and decreasing in blood pressure.
出处
《生物技术》
CAS
CSCD
1999年第1期16-20,共5页
Biotechnology
关键词
心钠素
乳猪
纯化
等电聚焦
HPLC
生物活性
cardionatrin. neonatal porcine
purification. IEF. RP-HPLC. biological active