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乳猪小分子心钠素的分离纯化和生物活性研究

ISOLATION, PURIFICATION AND BIOLOGICAL ACTIVE OF LOW MOLECULAR CARDIONATRIN FROM NEONATAL PORCINE
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摘要 乳猪心房组织提取液经分子膜截留,去除大分子,小分子部分经SePhPdexG-25凝胶分离,SP离子交换层析,RP-HPLCC18柱进一步纯化,获得了均一的心钠素,活性鉴定表明它有较高的利尿、利钠和降压活性。 The extraction of artrial tissue of neonatal porcine was ultrafiltered to remove largermolecular;; the lower molecular (Mw<10 000Da) was collected for isolation by sephadex G-25 gel;; purification by SP ion - exchange and further purification by RP - HPLC. Theproduct was tested by IEF;; showed that purified cardionatrin was acquired. It also showedhigh active of dilution;; natriuretic response;; and decreasing in blood pressure.
出处 《生物技术》 CAS CSCD 1999年第1期16-20,共5页 Biotechnology
关键词 心钠素 乳猪 纯化 等电聚焦 HPLC 生物活性 cardionatrin. neonatal porcine purification. IEF. RP-HPLC. biological active
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