摘要
原癌基因erbB-2的异常表达存在于人类多种肿瘤中,与肿瘤的发生、发展密切相关[1].我们曾构建了反义erbB-2逆转录病毒重组载体,将其转染存在该基因异常表达的人胃癌细胞系BGC-823,达到了特异抑制erbB-2表达、抑制瘤细胞恶性增殖并部分阻断...
DNA transfection,Nuclear Run On transcription assay were utilized to investigate the transcription rate of p21,p53 gene under EGF stimulation before and after the significant inhibition of erbB 2 proto oncogene(up to 65%)by its antisense retroviral recombinants.The results showed that after erbB 2 inhibition,EGF stimulation,significantly promoted the transcription of p21(up to 120%)and p53(up to 50%)gene in nuclei from human gastric cancer cells,which demonstrates that the expressional and functional changes of erbB 2 oncogene may be involved in the induction of p21,p53 gene by EGF.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1999年第1期154-156,共3页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金