摘要
细胞内的Ｏ６－甲基鸟嘌呤－ＤＮＡ甲基转移酶（ＭＧＭＴ）能够修复亚硝脲药物造成的ＤＮＡ损伤，阻止亚硝脲药物对肿瘤的杀伤作用，使细胞对亚硝脲药物产生耐药性．我们曾经构建了三个表达ＭＧＭＴ反义ＲＮＡ的逆转录病毒载体，导入对亚硝脲呈抗性的ＨｅＬａＳ３细胞，并...
Decrease of tumor cellular resistance to ACNU by retroviral mediated antisense RNA targeting 5′ region of O 6 methylguanine DNA methyltransferase(MGMT)mRNA was reported.It was found that after MGMT gene expression was depleted by antisense RNA,the tumor implanted in nude mice exhibited to be more sensitive to the killing effect of ACNU.It suggested that appropriate antisense RNA might deplete MGMT activity in human tumor cells and decrease drug resistance to ACNU.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1999年第1期157-158,共2页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金
