蓝藻OscilatoriaceaerDNA16S-23S基因间隔区的序列分析及其分类学意义

Sequence Analyses of rDNA Intergenic Spacer Region from Oscillatoriaceae(Cyanobacteria)and Its Taxonomic Significance

采用末端终止法对蓝藻类颤藻科Ｏｓｃｉｌａｔｏｒｉａｓｐ．ｒＤＮＡ１６Ｓ－２３Ｓ基因间隔区进行了序列测定，获得了Ｏｓｃｉｌａｔｏｒｉａｓｐ．ｒＤＮＡ基因间隔区４２７个核苷酸，其中包含１个异亮氨酸ｔＲＮＡ基因（ｔＲＮＡＩｌｅ）。并通过计算机联网从国际分子生物学数据弹库中获取颤藻科其它种的ｒＤＮＡ基因间隔区序列，通过比较分析，从分子水平对颤藻科Ｏｓｃｉｌａｔｏｒｉａｃｅａｅ属间的某些分类学问题进行了讨论，并根据序列中核苷酸差异值探讨了颤藻科属间界定的分子标准。提出了ｒＤＮＡ基因间隔区是良好的分子标记，可用于“赤潮”或“水华”

The sequence of rDNA Intergenic Spacer Region(ISR)was determined for the species Oscillatoria sp.(Cyanobacteria). The spacer sequences was 427bp in lengh exclusive of 16S and 23S rDNA coding regions,containing a Ile tRNA gene. Based on the analyses of the obtained sequences and the sequences from other three representative species of Oscillatoriaceae, and two species of Microcystis , the evolutionary affiliation among the genera of Oscillatoriaceae was discussed.In addition, the results showed that rDNA Intergenic Spacer Region can be served as a criteria for the identification or the classification of Cyanobacteria within the genus or intraspecies, which may be a useful genetic marker at the species or population level and may be one of the most suitable regions in the rRNA gene cistron for preparing DNA probes specific to Cyanobacteria species because of the high divergence of this region.