RP-HPLC同时测定管花肉苁蓉中4种苯乙醇苷的含量

RP-HPLC simultaneous determination of four phenylethanoid glycosides in Cistanche tubulosa (Schrenk) Wight

目的：建立RP-HPLC同时测定管花肉苁蓉药材中松果菊苷、管花苷A、毛蕊花糖苷和异毛蕊花糖苷含量的方法。方法：采用Megres-C18（250mm×4.6mm,5μm）色谱柱,柱温30℃;流动相为甲醇（A）-0.1%甲酸（B）,梯度洗脱（0min,30%A;30min,40%A;40min,45%A）,流速1.0mL·min-1;检测波长330nm。结果：在上述色谱条件下,松果菊苷、管花苷A、毛蕊花糖苷和异毛蕊花糖苷获得良好分离;进样量分别在0.732~11.7μg（r=0.9991）、0.216~3.45μg（r=0.9995）、0.209~3.35μg（r=0.9991）、0.151~2.42μg（r=0.9993）范围内呈良好的线性关系;平均加样回收率（n=9）分别为101.8%,98.8%,101.1%,99.9%;精密度试验的RSD小于0.65%;重复性试验的RSD小于2.4%。结论：该方法简便、准确,重复性好,为管花肉苁蓉药材的质量控制提供可借鉴的分析方法。

Objective：To simultaneous determine the contents of echinacoside,tubuloside A,acteoside and isoacteoside in rhizome of Cistanche tubulosa（Schrenk）Wight by RP-HPLC.Methods：An external standard method by RP-HPLC with Megres-C18（250 mm×4.6 mm,5 μm）as fixed phase at the temperature 30 ℃.The mobile phase consisted of methanol（A）-0.1% aqueous methanoic acid（B）with the gradient elution（0 min,30%A;30 min,40%A;40 min,45%A）,the flow rate：1.0 mL·min-1.The Absorbance was monitored 330 nm.Results：Echinacoside,tubuloside A,acteoside and isoacteoside were well separated by this method.Linearities of echinacoside,tubuloside A,acteoside and isoacteoside were good in the ranges of 0.732-11.7 μg（r=0.9991）,0.216-3.45 μg（r=0.9995）,0.209-3.35 μg（r=0.9991）,0.151-2.42 μg（r=0.9993）,respectively.The average recoveries（n=9）of four phenylethanoid glycosides were 101.8%,98.8%,101.1%,99.9%,respectively.All of RSDs of precision（n=6）and repeatability（n=6）were less than 0.65% and 2.4%.Conclusion：The method is simple,accurate,reproducible,and can be used for quality control of Cistanche tubulosa（Schrenk）Wight.