桑黄深层发酵胞外酶活性的测定与分析

Analysis of extracellular enzyme activities of Phellinus baumii by submerged fermentation

以不同的桑黄菌株为材料,通过胞外淀粉酶、滤纸纤维素酶、羧甲基纤维素酶、漆酶、愈创木酚酶、多酚氧化酶活性测定,筛选较优菌株,并对该菌株胞外酶活性进行动态分析。结果表明:菌株ZKY的淀粉酶、羧甲基纤维素酶、滤纸纤维素酶的活性均高于其它菌株,木质素降解酶中愈创木酚酶和多酚氧化酶活性较低,但胞外蛋白质含量明显高于其它菌株;ZKY对碳水化合物利用顺序为淀粉、纤维素、木质素,其淀粉酶、羧甲基纤维素酶、滤纸纤维素酶、漆酶,活性高峰分别出现在发酵的第4、6、8d,愈创木酚酶和多酚氧化酶在发酵过程中均出现两个峰,第一个峰出现在发酵的第10d,之后均有第二个峰出现。

The activity of amylase, cellulase, carboxymethyl cellulose, laccase, guaiacol oxidase, polyphenol oxidase of Phellinus baumfi under submerged culture were determined, and based on this, excellent strain was filtrated. Dynamic variation of extracellular enzyme activity of the excellent strain was investigated.The results showed that the activity of amylase, carboxymethyl cellulose and cellulase of ZKY were the higher than others, and the activity of guaiacol oxidase and polyphenol oxides were low, but the content of extracellular protein of ZKY was evidently higher than others, which showed ZKY was a excellent strain. The maximum activity of amylase, cellulose and laccase appeared on the 4, 6, 8d respectively during the fermentation. The activity of guaiacol oxidase and polyphenol oxidase showed two peak values,of which the first peak appeared on the 10d, and the second peak appeared subsequently.