LC-MS在体内药物分析方法学研究中常见的问题及其对策

LC-MS in biomedical analysis:Common issues and counterplan

液相色谱-质谱联用技术(liquid chromatography-mass spectrometry,LC-MS)已成为体内药物分析及其相关研究领域中不可或缺的工具.虽然该技术具有高选择性、高灵敏度以及高通量等特点,但在体内药物分析方法学研究中,仍然会面临诸如待测物需衍生化、复方制剂体内多组分同时测定时高浓度组分的质谱响应饱和、方法专属性误判、基质效应、残留效应等一系列挑战和问题,本文综述了这些常见问题的相应对策.

Liquid chromatography-mass spectrometry（LC-MS） is currently the method of choice for the biomedical analysis.The advantages of this technique include high specificity,sensitivity and throughput.However,in practical terms,we still face many challenges and/or problems,such as derivatization,MS response saturation to high content components in simultaneous determination of the multi-component,false-positive results of specificity,matrix effect and carry-over effect.In this review,these issues and their counterplan in bioanalytical LC-MS method development and validation are discussed and illustrated with some examples.