超声波复合酶法提取桑黄多糖研究

Ultrasonic Pretreatment Followed by Enzymatic Hydrolysis for Extraction of Polysaccharides from Fermented Mycelia of Phellinus linteus

采用单因子分析和正交试验,以桑黄菌丝体提取物中多糖得率为指标,对超声波复合酶法中影响多糖提取效果的主要因素进行研究。结果表明:超声波提取优化工艺条件为超声处理时间20min、料液比1:25(g/mL)、功率500W,在此基础上提取多糖得率为3.356%,在超声波优化结果基础上,进一步进行复合酶法处理,酶解最佳提取条件是pH6.5,酶解温度50℃,纤维素酶添加量2.5%、果胶酶添加量2.5%、蛋白酶添加量1%,酶解时间120min,多糖得率为6.619%,由此可见,超声波和复合酶法双重处理提取桑黄多糖是一种有效的提取方法,适合大规模生产运用。

To develop an optimal procedure based on ultrasonic pretreatment followed by enzymatic hydrolysis for the extraction of polysaccharides from the fermented mycelia of Phellinus linteus, single factor and orthogonal array design methods were used to deal with the effects of crucial ultrasonic pretreatment and hydrolysis parameters on polysaccharide yield. Results showed that the optimization of ultrasonic pretreatment led to 3.356% polysaccharide yield, in which, the optimal values of ultrasonic power, pretreatment time and material/liquid ratio were determined to be 500 W, 20 min and 1：25 （g/mL）, respectively. The optimization of enzymatic hydrolysis leading to 6.619% polysaccharide yield was achieved through the combinatorial use of cellulase at 2.5% dosage, pectinase at 2.5% dosage and neutral proteinase at 1% dosage for the hydrolysis for 120 min at pH 6.5 and 50 ℃. The combination of ultrasonic pretreatment and enzymatic hydrolysis may provide a good idea for the large-scale production of polysaccharides from the fermented mycelia of Phellinus linteus.