高效液相色谱法测定蜂王幼虫中辅酶Q_(10)含量

HPLC Determination of Coenzyme Q_(10) in Queen Bee Larvae

建立一种用高效液相色谱测定蜂王幼虫中辅酶Q10含量的方法。蜂王幼虫样品研磨均质后,经无水乙醇-正己烷液/液萃取,提取液经浓缩后,无水乙醇定容。以甲醇为流动相,采用XBridgeshieldRP18柱分离,在紫外检测器275nm波长处对样品中的辅酶Q10进行测定。辅酶Q10在12min内得到较好的分离,方法线性良好,回收率为95.0%～101.8%,相对标准偏差(RSD)小于8%,检出限为0.23mg/L。该方法操作简单、准确、可靠、灵敏度高,适用于测定蜂王幼虫中辅酶Q10的含量,已被用于分析一些实际样品,调查辅酶Q10在蜂王幼虫样品中的含量情况。

A high performance liquid chromatographic （HPLC） method was established to determine coenzyme Q10 in queen bee larvae. Samples were ground and extracted with absolute ethanol under the assistance of ultrasonic and the resulting extract was then reextracted with n-hexane. The n-hexane layer was finally condensed by rotation evaporation and rediluted with absolute ethanol. The chromatographic separation was achieved on an XBridge shield RP18 column using methanol as a mobile phase prior to UV detection at 275 nm wavelength. Results showed that a good separation of coenzyme Q10 was observed within 12 min. The developed standard curve exhibited good linearity. Average recoveries for coenzyme Q10 in a real sample spiked at 3 levels, each of which was performed in 6 replicates, were within the range of 95.0% to 101.8%, with a RSD of less than 8%. The limit of detection for coenzyme Q10 was 0.23 mg/L. The analytical method proved to be simple, accurate, reliable and sensitive so as to be most suitable for the determination of coenzyme Q10 in queen bee larvae. Satisfying results from the analysis of coenzyme Q10 in some real samples by the method were obtained.