丙酮酸钙对大鼠脂肪酸代谢的影响

EFFECT OF CALCIUM PYRUVATE ON FATTY ACID METABOLISM IN RATS

目的研究丙酮酸钙对大鼠脂肪酸代谢的影响及其机制。方法选取32只体重150g左右的雄性SD大鼠随机分成对照和丙酮酸钙低、中和高剂量(calcium pyruvate:0、100、500和1000mg/(kg.d)),灌胃方式给予,持续4w后处死并采样。用生化分析仪测定大鼠血脂和血糖指标,毛细管气相色谱法测定脂肪、肝脏和肌肉组织中脂肪酸C16:0、C18:0、c9-C18:1、c9,c12-C18:2、α-C18:3、C20:4含量,RT-PCR法测定肝脏中ACCαmRNA表达水平。结果实验组的体重和食物利用率显著低于对照组(P<0.05)。高、中剂量组的总胆固醇含量显著低于对照组(P<0.05);各组间的血糖和甘油三酯含量变化不明显。脂肪酸测定表明脂肪中除C18:0,肝脏中除C18:0和c12-C18:2无统计意义,其余脂肪酸含量与对照组比显著减少(P<0.05);背长肌中的脂肪酸含量变化不明显。肝脏中高剂量组ACCαmRNA表达水平显著低于与对照组(P<0.05)。结论丙酮酸钙能控制体重增长,降低脂肪酸含量,可能是通过调节肝脏ACCαmRNA转录水平和增加机体能量消耗实现的。

Objective To investigate the effects of calcium pyruvate supplementation on body weight gain and fatty acid metabolism in rats.Method Thirty-two 150 g male SD rats were randomly divided into four groups：control group,low,middle and high dose calcium pyruvate group （0,100,500 and 1000mg/（kg.d））.Calcium pyruvate was administered intragastrically to rats-for 4 w,and then the rats were killed and blood and tissue samples were collected.Serum lipids and blood glucose were determined by biochemistry analyzer.Analysis of fatty acid composition of adipose tissue,liver tissue and musculature was determined by capillary gas chromatography and mRNA expressions of ACC-α in liver by RT-PCR.Result At the end of the experiment,the body weight and food conversion efficiency of rats in all experiment groups were significantly lower than that of control group.TG of high and middle dose group were obviously lower than that of control group.TC and blood glucose concentration were not statistically different among different groups.contents of C16：0,c9-C18：1,c9,c12-C18：2,α-C18：3 and C20：4 in the adipose tissue were significantly decreased in the experiment groups,but the content of C18：0 was not statistically different on fatty acid composition of musculature in rats.Fatty acid composition of liver tissue was remarkably lower than that of the control group,except C18：0 and c12-C18：2.mRNA expression level of ACC in liver of high dose group was significantly decreased （P0.05）.Conclusion Calcium pyruvate might have effect on hepatic lipid metabolism through regulation of ACC at the transcriptional level and increasing consumption of energy.