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脑内皮细胞趋化因子受体5参与β淀粉样蛋白引起的T细胞跨内皮迁移 被引量：1

Chemokine Receptor 5 of Human Brain Microvascular Endothelial Cells Takes Part in Amyloid beta Induced T Lymphocytes Transendothelial Migration
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摘要 目的利用血-脑屏障的体外模型,分析脑内皮细胞上的趋化因子受体5(CCR5)在β淀粉样蛋白(Aβ)引起的T细胞跨内皮迁移过程中的作用。方法培养人脑微血管内皮细胞(HBMEC),建立体外血-脑屏障模型,分析T淋巴细胞跨内皮迁移的能力。结果 Aβ能够促进T淋巴细胞穿过体外血-脑屏障模型,HBMEC中CCR5过表达能够促进T淋巴细胞穿过体外血脑屏障模型,HBMEC中CCR5缺失突变能够抑制T淋巴细胞穿过体外血-脑屏障模型。结论 HBMEC中CCR5参与Aβ引起的T淋巴细胞的跨内皮迁移。 Objective To investigate the function of chemokine receptor 5（CCR5）in human brain microvascular endothelial cells（HBMEC）during T lymphocytes transendothelial migration induced by amyloid beta（Aβ）.Methods The in vitro model of blood-brain barrier was established with cultured HBMEC monolayer.The ability of T lymphocytes transendothelial migration induced by Aβ was analyzed by Transwell and Millicell-ERS endothelia volt-ohmmeter.Results Aβ could promote T lymphocytes to migrate through the HBMEC monolayer.Over-expression of CCR5 in HBMEC promoted T lymphocytes to migrate through HBMEC monolayer.Expression of CCR5 mutant in HBMEC inhibited the transendothelial migration of T lymphocytes.Conclusion CCR5 of HBMEC is involved in T lymphocytes transendothelial migration induced by Aβ.
作者 朱莉 李曼 赵伟东 陈誉华
机构地区 中国医科大学基础医学院发育生物学教研室
出处 《中国医科大学学报》 CAS CSCD 北大核心 2010年第6期410-412,431,共4页 Journal of China Medical University
基金 国家自然科学基金资助项目(30700279)
关键词 Β淀粉样蛋白 趋化因子受体5 人脑微血管内皮细胞 T淋巴细胞 跨内皮迁移 amyloid beta chemokine receptor 5 brain microvascular endothelial cell T lymphocytes transendothelial migration
分类号 Q786 [生物学—分子生物学]
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参考文献7

  • 1Town T,Tan J,Flavell RA,et al. T-cells in Alzheimer's disease [J]. Neuromol Med, 2005,7 ( 3 ) : 255-264.
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二级参考文献13

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中国医科大学学报
2010年 第6期

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