二次启动法培养无凝结核的硫酸盐还原颗粒污泥

Cultivation of No Condensation Nuclei Sulfate Reducing Granular Sludge with Two-stage Start Method

采用自制EGSB反应器,通过二次启动法培养无凝结核的硫酸盐还原颗粒污泥。第一阶段为非硫酸盐还原培养阶段,在进水有机负荷为10.20kgCOD/(m3.d)、碱度为3000mg/L、回流比为3.6∶1时,可初步培养出厌氧颗粒污泥,其以黑色为主,直径为1～2mm,此时对COD的去除率可达90%以上,出水pH值稳定在6.5～7.5之间,ORP值为-250mV左右;第二阶段为硫酸盐还原阶段,在保持其他条件不变的情况下添加1000mg/L的硫酸盐,成功培养出硫酸盐还原颗粒污泥,EGSB反应器对COD和硫酸盐的去除率均稳定在70%左右,出水中的硫化物含量为25mg/L、pH值为6.8～7.5、ORP值为-300～-350mV。硫酸盐还原颗粒污泥呈深黑色、椭球状,无凝结核,生长有大量微生物,以杆菌及弧菌为主,粒径主要分布在2～3mm的范围内。

No condensation nuclei sulfate reducing granular sludge （SRGS） was cultivated in an EGSB reactor through two-stage start method. At the first non-sulfate reducing stage, the anaerobic granular sludge can be cultivated when the influent organic loading is 10.20 kgCOD/（m3 ·d） , alkalinity is 3 000 mg/L and reflux ratio is 3.6 ： 1. The color of granular sludge is black, and the diameter is 1 to 2 ram. At that time, the removal rate of COD can reach above 90% , pH value is 6.5 to 7.5, and ORP is about -250 mV. At the second sulfate reducing stage, 1 000 mg/L sulfate is added, and the SRGS is successfully cultivated when keeping other conditions constant. The removal rates of COD and sulfate are all about 70%. In the effluent, the content of sulfate is 25 rag/L, pH value is 6.8 to 7.5 and ORP is - 300 to - 350 inV. The SRGS is a dark black oval and no condensation nuclei. A large number of microorganisms grow in the SRGS, mainly the bacillus and vibrio. The diameter of SRGS is 2 to 3 ram.