正交偏振多光谱成像术应用于活体微循环观察研究

Observation of and Research on Intravital Microcirculation Use of Orthogonal Polarization Multi-Spectral Technique

构建一种小型、简洁的活体微循环多光谱成像系统,将正交偏振光谱成像术OPS Imaging及液晶可调谐滤光片LCTF应用于活体微循环多光谱成像。LCTF具有较高的成像质量、较低的功耗、且没有移动部件和图像移动,能够连续、随机地在可见-近红外波段上快速实现波长的任意调谐。利用该系统,对活体裸鼠的耳廓采用检偏角为90°(正交偏振方式)与0°两种方式进行了试验,获取了裸鼠耳廓微血管的多光谱图,得出采用正交偏振成像方式的图像的对比度及图像细节均优于检偏角为0°时的图像。试验验证了正交偏振多光谱成像技术能增强活体微循环观察效果。

A smalh simple intravital microcirculation multi-spectral imaging system was constructed. Orthogonal polarization spectral （OPS） imaging and liquid crystal tunable Jilter （LCTF） were used for intravital mierocirculation multi-spectral imaging. LCTF has excellent imaging quality and low-power loss, without moving parts and image shift. It can provide continuously, rapid and random-access selection of any wavelength in the visible to near-infrared range. An ear of nude mouse was adopted as experiment specimen- The multi-spectral images of ear microvessel of the nude mouse were acquired by this imaging system in two ways （detection polarization angle 90° and 0°）. Compared with detection polarization angle 0° way the images acquired by orthogonal polarization spectral imaging way（detection polarization angle 90°） had better contrast and more details. It is concluded that the experiment has proved that the orthogonal polarization multi-spectral technique can enhance the effect of observation of intravital microcirculation.