利用深黄被孢霉发酵生产油脂的初步研究

Preliminary Study on the Mortierella isabellina Fermentating to Produce Lipid

为提高微生物油脂产量,以产油微生物深黄被孢霉为试验菌株,采用单因素试验设计,通过摇瓶培养,研究了产脂培养基中碳源、氮源种类及其浓度、接种量、初始pH值、无机盐离子对菌体生长和油脂积累的影响,确定了深黄被孢霉摇瓶发酵产油脂的优化培养条件为：葡萄糖100 g.L-1,酵母粉3.0 g.L-1,接种量为20%,pH值为5.0-6.0,硫酸镁（MgSO4.7H2O）0.5 g.L-1,磷酸二氢钾（KH2PO4）2.0 g.L-1。在优化培养条件下菌体生物量为20.62 g.L-1,油脂含量为43.02%,油脂产量为8.87 g.L-1。

To improve the yield of microbial lipids,the lipids production was studied by using the fungus Mortierella isabellina.On the basis of the single factor experiment,the effect of various carbon and nitrogen sources and their concentrations,inoculum size,initial pH and inorganic ions on mycelium growth and accumulation of lipid yield in shaker culture was studied.The results showed that the optimal culture condition were as follows： glucose 100 g·L-1,yeast extract 3.0 g·L-1,inoculum size 20%,initial pH 5.0～6.0,MgSO4·7H2O 0.5 g·L-1,KH2PO4 2.0 g·L-1.Under the optimum culture condition,the biomass yield was 20.62 g·L-1,lipid content was 43.02%,the lipid yield was 8.87 g·L-1.