水稻无叶枕基因克隆及应用研究

CLONING AND APPLICATION OF A RICE LIGULELESS GENE

本研究报道由早籼品种"广陆矮4号"经60Coγ射线辐射诱变产生的水稻无叶枕突变体Oslg1-3。该水稻无叶枕性状受单隐性基因控制,通过图位克隆的方法将基因定位在第4号染色体,并最终克隆出该基因。除了无叶枕以外,Oslg1-3突变体的叶角即叶片与叶鞘之间的夹角也很小,因而叶片直立,可以用来培育叶片直立型水稻新品种。田间试验分析表明,经过遗传改良的Oslg1-3品系在合理密植的条件下能够显著提高水稻的产量,显示出该突变体材料在水稻育种实践中具有重要的应用价值。

This paper reported a rice liguleless mutant,Oslg1-3,which was induced from early indica rice variety ＂ Guanglu’ai 4＂by 60Co γ-rays irradiation. The trait of ligulelessness was inherited as a single recessive gene model,and the corresponding gene OsLG1 was localized to chromosome 4 and isolated via mapping-based cloning method. Besides the absence of ligule,the leaves of the mutant were erect due to low degree of bending between leaf blade and sheath,so the mutant could be utilized to breed new rice varieties with erect leaf. The analysis of field production indicated that the genetically improved Oslg1-3 lines could significantly increase grain yield through dense planting,thus demonstrated the mutant was valuable in rice breeding.