绢丝昆虫樟蚕线粒体全基因组克隆及序列分析

CLONING AND SEQUENCING ANALYSIS OF THE COMPLETE MITOCHONDRIAL GENOME OF Eriogyna pyretorum

采用Long-PCR方法,克隆并测定了樟蚕(Eriogyna pyretorum)线粒体基因组全序列。樟蚕线粒体基因组全序列为15,327bp,基因组碱基组成为39.17%A,11.55%C,7.63%G,41.65%T,共有13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和1个AT富含区(A+T-rich区)。樟蚕线粒体基因组结构与鳞翅目其他昆虫相同。除cox1和cox2基因外,其他蛋白编码基因含有典型的ATN起始密码子;13个蛋白质编码基因中,有11个使用TAA作为终止密码子。预测了22个tRNA基因的二级结构,发现除trnS1(AGN)缺少DHU臂外,其他的tRNA具有典型的三叶草结构。

The complete mitochondrial genome of E. pyretorum was cloned using long-PCR amplification. The mitochondrial genome of E. pyretorum was 15,327bp. The base compositions included 39. 17% A,11. 55% C, 7. 63% G,41. 65% T,and consisted of 13 protein coding gene （PCGs）,2 rRNAs and 22 tRNA genes and one A ＋ T- rich region. All PCGs of the E. pyretorum mitochondrial genome started with a typical ATN codon,except for cox1 and cox2. The usual TAA termination codon was found for eleven PCGs. The arrangement of PCGs was the same as that found in other sequenced lepidopteran insect. However,cox1 and cox2 genes had an incomplete termination codon （T）. With respect to the secondary structures of 22 tRNA genes,the absence of DHU arm of trnS1 （ AGN） was observed in E. pyretorum.