PEG与SNP对低温下马铃薯试管苗相关生理指标的影响

EFFECTS OF PEG-6000 AND EXOGENOUS NITRIC OXIDE DONOR SNP ON THE RELATED PHYSIOLOGICAL INDICES OF POTATO SEEDLINGS In vitro UNDER LOW TEMPERATURE

研究了在4℃低温胁迫下,5%聚乙二醇PEG-6000和0.10mmol/LSNP分别处理,5%PEG-6000与0.10mmol/LSNP同时处理对陇薯3号马铃薯试管苗的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和对丙二醛(MDA)、可溶性蛋白、游离脯氨酸及叶绿素含量的影响。结果表明,4℃低温下,PEG-6000和0.10mmol/LSNP都可以胁迫试管苗,导致其酶活性、MDA、可溶性蛋白、脯氨酸和叶绿素含量都有所上升,其中0.10mmol/LSNP可以显著提高上述各项生理指标,并在和5%PEG-6000共同处理时缓解PEG造成的渗透胁迫。

In this paper potato seedlings of Longshu Ⅲ in vitro were treated with 5% of polyethylene glycol（PEG-6000）, 0. 10mmol /L sodium nitroprussede （ SNP）,and treated with 5% PEG-6000 and 0. 10mmol /L SNP,the effects of treatments, on superoxide dismutase （ SOD ）,peroxidase （ POD ） and catalase （ CAT ） activity, contents of malondidehyde（ MDA）,soluble protein,proline and chlorophyll of the seedlings under low temperature of 4℃ were studied. Results indicated that,under 4℃ both PEG-6000 and 0. 10mmol /L SNP treatments could cause the increase of their enzymatic activity and the contents of MDA,soluble protein,free proline and chlorophyll in potato seedlings. Low concentration of SNP can significantly enhance all measured physiological index,and could alleviate the osmotic stress at combination treatment with the 5% PEG-6000.