利用耳组织、血液、牛奶样品检测牛病毒性腹泻病毒的比较

Comparison among Detection of BVDV Infection by Ear Notch Tissue,Blood and Milk in Dairy Cattle

牛病毒性腹泻病以发热、口腔及消化道粘膜糜烂、腹泻为主要症状,可引起怀孕母牛流产或产畸型胎儿,给养牛业造成巨大的经济损失,该病已成为当前严重危害我国养牛业的传染病之一。由于大部分感染BVDV的牛不表现特征性的临床症状和病理变化,而且引起腹泻和消化道黏膜糜烂或溃疡的疾病很多,所以确诊需进行实验室检查。目前实验室检测BVDV的主要方法是利用美国IDEXX公司ELISA诊断试剂盒,通过BVDV抗原和抗体的检测区别牛群持续感染牛。用于检测BVDV的样品种类有许多种,绝大多数是采集血液(血清)或牛奶样品进行检测。本试验对奶牛进行耳组织采样,同时采集血液和牛奶,用三种样品在相同条件下同时检测BVDV抗原,用血清和牛奶样品检测抗体,比较样品的检测结果。结果表明,这三种样品检测结果具有一致性,且耳组织采样更宜于大面积筛查。

Bovine viral diarrhea is an i mportant viral disease of cattle.It cause gastrointestinal,respiratory,and reproductive disease including abortion,abnormal fetus,fever,mouth and digestive canals ulcers,diarrhea,infertility,and brought on enormous economical loss in dairy industry.As most of cattle infected with BVDV did not show clinical symptoms and pathological features,and many infectious cattle have diarrhea and digestive canals ulcers or erosion,so it needs to carry on laboratory diagnosis.It is the major method to make out persistently infected cattle in laboratory currently via antigen and antibody detection by US IDEXX ELISA test kit.It has many kinds of samples to detect BVDV,most of them are blood（sera） or milk.This study collected ear notch tissue,blood and milk as samples from cattle,then detect BVDV antigen at the same condition with these three kinds samples,detect BVDV antibody with sera and milk samples.This study compare the consistensy of results of the three kinds of samples.The result showed that the consistensy of the results,and ear notch samples is more suitable for lage-scale screening.