期刊文献+

用石英晶体微天平法研究聚乳酸的酶降解过程 被引量:3

Enzymatic Degradation of PLA by a Quartz Crystal Microbalance
下载PDF
导出
摘要 利用石英晶体微天平(QCM)探讨影响聚乳酸降解速率的不同因素。聚乳酸的酶降解分两步进行:酶吸附到聚乳酸的表面和聚乳酸在酶作用下的降解。聚乳酸的降解初速率随酶加入量的增加而增大,但达到一定的加入量之后,降解初速率接近一个常数;当pH为7~9时,聚乳酸的降解初速率随pH的增大而增大;当温度为25~40℃时,降解初速率随温度的升高而增大。 The kinetics of the enzymatic degradation of PLA is investigated by quartz crystal microbalance(QCM).There are several important factors which affect the enzymatic degradation of PLA by proteinase K.The enzymatic degradation of PLA by proteinase K is a two-step process.The first step is adsorption of the enzyme on the surface of the substrate and the second step is degradation of PLA by proteinase K.The initial degradation rate increases with the increasing enzyme concentration and attains to be constant after the enzyme concentration approaches a certain value.The initial degradation rate increases with the increasing pH in the range of pH from 7 to 9.The initial degradation rate increases with increasing temperature in the range of temperature from 25 ℃ to 40 ℃.
出处 《华东理工大学学报(自然科学版)》 CAS CSCD 北大核心 2010年第3期389-394,共6页 Journal of East China University of Science and Technology
关键词 聚乳酸 酶降解 动力学 石英晶体微天平 PLA enzymatic degradation kinetics quartz crystal microbalance
  • 相关文献

参考文献17

  • 1Tokiwa Y,Calabia B P. Biodegradability and biodegradation of poly(lactide)[J]. Appl Microbiol Biotechnol, 2006,72 (2) :244 -251.
  • 2McDonald R T, McCarthy S P, Gross R A. Enzymatic degradability of poly(lactide) : Effects of chain stereochemis try and material crystallinity[J]. Macromolecules, 1996,29 (23) :7356-7361.
  • 3Moon S I, Urayama H, Kimura Y. Structural characteriza tion and degradability of poly(L lactic acid)s incorporating phenyl-substituted a-hydroxy acids as comonomers[J]. Macromol Biosci, 2003,3(6) :301-309.
  • 4Tsuji H, Ishizaka T. Porous biodegradable polyesters: 3. Preparation of porous poly(e-caprolactone) films from blends by selective enzymatic removal of poly(L-lactide)[J]. Macrotool Biosci, 2001,1(2) :59-65.
  • 5Cai H, Dave V, Gross R A, etal. Effects of physical aging, erystalllnity, and orientation on the enzymatic degradation of poly(lactic acid)[J]. J Polym Sci Part B: Polym Phys, 1996, 34,2701-2708.
  • 6Iwata T, Doi Y. Morphology and enzymatic degradation of poly(L-lactic acid) single crys[J]. Macromolecules, 1998,31 (8) : 2461-2467.
  • 7Tsuji H, Miyauchi S. Poly(lactide): VI. Effects of crystallinity on enzymatic hydrolysis of poly(L lactide) without free amorphous region[J]. Polym Degrad Stab, 2001,71(3):415- 424.
  • 8Tokiwa Y, Suzuki T, Ando T. Synthesis of copolyamideesters and some aspects involved in their hydrolysis by lipase [J]. J ApplPolym Sci,1979,24(7):1701-1711.
  • 9Tokiwa Y, Suzuki T. Hydrolysis of copolyesters containing aromatic and aliphatic ester blocks by lipase[J]. J Appl Polym Sci, 1981,26(2) :441-448.
  • 10Li S, McCarthy S. Influence of crystallinity and stereo chemistry on the enzymatic degradation of poly(lactide)s[J]. Macromolecules, 1999,32(13) : 4454-4456.

同被引文献37

  • 1瞿丽曼.聚乳酸在国内外包装应用中的发展趋势[J].化工新型材料,2007,35(S1):100-102. 被引量:2
  • 2Gong Feirong,Cheng Xiaoyan, Wang Shanfeng, et al. Biode gradable comb dendritic tri-block copolymers consisting of poly(ethylene glycol) and poly ( L lactide) : Synthesis, charac terizations, and regulation of surface morphology and cellresponses[J]. Polymer,2009,50(13) :2775-2785.
  • 3Pomorska A,Yliniemi K,Wilson B P,et al. QCM study of the adsorption of polyelectrolyte covered mesoporous TiO2 nanocontainers on SAM modified Au surfaces[J]. Journal of Colloid and Interface Science,2011,362( 1):180-187.
  • 4Yeon K B,Hyung H K,Deog S P,etal. A new concept for efficient sensitivity amplification of a QCM based immunosen sor for TNF-a by using modified magnetic particles under applied magnetic field[J]. Journal of he American Chemical Society,2011,32(12) =4215-4220.
  • 5Soo Hong Lee,Soo Hyun Kim, Yang Kyoo Han,et al. Syn thesis and degradation of end group-funetionalized polylaetide [J]. Journal of Polymer Science: Part A. Polymer Chemis try,2001,39(7) =973-985.
  • 6Zhao Youliang, Shuai Xintao, Chen Chuanfu, et al. Synthesis and characterization of star shaped poly (L-lactide)s initiated with hydroxyl terminated poly(amidoamine) (PAMAM-OH) dendrimers[J]. Chemistry of Materials, 2003,15 (14) : 28:36 2843.
  • 7Cai H,Dave V,Gross R A,etal. Effects of physical aging, crystallinity,and orientation on the enzymatic degradation of poly(lactic acid)[J]. Journal of Polymer Scienee Part B. Polymer Physics, 1996,34(16) : 2701-2708.
  • 8Iwata T, Doi Y. Morphology and enzymatic degradation o{ poly(L-lactic acid) single crys[J]. Macromolecules, 1998,31 (8) 2461 2467.
  • 9Tsuji H,Miyauchi S. Poly (lactide) VI. Effects of crystallin- ity on enzymatic hydrolysis of poly (L-lactide) without free amorphous region [J]. Polymer Degradation and Stability, 2001,71(3) :415-424.
  • 10LEE H,AHN S,CHOI H,et al. Fabrication,Characterization,and in Vitro Biological Activities of Melt-electrospun PLAMicro/Nanofibers for Bone Tissue Regeneration[J]. J MaterChem B,2013(1):3670-3677.

引证文献3

二级引证文献32

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部