期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

三苯氧胺对人脑胶质瘤细胞内游离钙离子水平的影响 被引量：2

Effects of tamoxifen on the cytosolic free calcium level in human SHG-44 cells
下载PDF
导出
摘要 以不同浓度的三苯氧胺(TAM)处理SHG-44细胞后,用流式细胞仪检测细胞凋亡率和激光共聚焦显微镜测定细胞内游离Ca2+水平,探讨TAM对人脑胶质瘤细胞内游离钙离子水平的影响。结果表明:当细胞外有Ca2+时,TAM使细胞内Ca2+浓度迅速升高并呈剂量依赖性关系,其浓度能维持在较高水平。当细胞外无Ca2+时,TAM呈剂量依赖性地使胞内Ca2+浓度缓慢升高,其升高的幅度和速度低于细胞外有Ca2+组。说明TAM能促进SHG-44细胞胞外Ca2+内流和胞内钙库释放Ca2+,提高细胞内游离Ca2+浓度;TAM诱导的细胞凋亡与提高细胞内游离Ca2+浓度有关。 To investigate the effects of tamoxifen on the cytosolic free calcium level, the apoptotic rate was measured by flow cytometry assay and the cytosolic free calcium level was determined by laser confocal microscope assay after treating human glioma cell SHG-44 with various concentration of tamoxifen. Result showed that, in medium containing extracellular Ca^2＋ , tamoxifen promptly increased cytosolic free calcium level in a dose-dependent manner, and afterwards it remained at a high level. Although tamoxifen also increased cytosolic free calcium level in a dose-dependent manner without extracellular calcium, it showed much slower and less. In conclusion, tamoxifen is likely to increase cytosolic calcium by releasing intracellular calcium and/or causing extracellular calcium influx. Apoptosis induced by tamoxifen may be related to its ability to increase the cytosolic free calcium level in human glioma cell SHG-44.
作者 宁萍 刘芬菊 李冰燕
机构地区 苏州大学医学部
出处 《扬州大学学报（农业与生命科学版）》 CAS CSCD 北大核心 2010年第1期20-23,共4页 Journal of Yangzhou University：Agricultural and Life Science Edition
基金 国家自然科学基金资助项目(30870585) 苏州大学青年基金资助项目(Q3126624)
关键词 三苯氧胺 脑胶质瘤细胞 钙离子 calcium apopotosis glioma
分类号 R730.5 [医药卫生—肿瘤] R739.4 [医药卫生—肿瘤]
  • 相关文献

参考文献11

  • 1宁萍,刘芬菊,姜建平.三苯氧胺对人脑胶质瘤细胞SHG-44增殖的影响[J].苏州大学学报（医学版）,2009,29(5):852-854. 被引量：2
  • 2Markman M, Iseminger K A, Hatch K D. Tamoxifen in platinum-refractory ovarian cancer:a gynecologic oncology group ancillary report [J]. Gynercol Oncol, 1996, 62: 4-6.
  • 3宁萍,刘芬菊,江家贵,姜建平.Tamoxifen对人脑胶质瘤细胞SHG-44辐射敏感性的影响[J].中国辐射卫生,2009,18(1):15-16. 被引量：1
  • 4Chen Q M, Tu V C. Apoptosis and hean failure: mechanisms and therapeutic implications [J].Am J Cardiovasc Drugs, 2002, 2(1): 43- 57.
  • 5Strasser A, Oconnor L, Dixit V M. Apoptosis signaling [J]. Annual Review of Biochenistry, 2000, 69.. 217 -245.
  • 6Kobayashi S, Yamsashita K, TAkeoka T, et al. Calpain-mediated X-linked inhibitor of apoptosis degradation in neutrophil apoptosis and it's impairment in chronic neutrophilic leukemia [J]. J Biol Chem, 2002, 277(37): 3968- 3977.
  • 7Kim M J, Jo D G, Hong G S, et al. Calpain dependent cleavage of eain/cabinl activates calcineurin to mediate calcium-triggered cell death [J]. Proc Natl Acad Sci USA, 2002, 99(15): 9870 -9875.
  • 8Shibasaki F, Hallin U, Unchino H. Calcineurin as a muhifunctional regulator [J]. J Biochem(Yokyo), 2002, 131(1) : 1-15.
  • 9Heller B, Wang Z Q. Inactivation of the poly (ADP-ribose) polymers gene affects oxygen radical and nit ric oxide toxicityinislet cells [J].J BiolChem, 1995, 270(9): 11176-11180.
  • 10Orrenius S. Mechanisms of oxidative cell damage [C]// Poli G, Albano E, Dianzani M, et al. From BasicScience to Medicine. Basel: Birkhanser Verlag, 1993: 47-64.

二级参考文献15

  • 1谷铣之,殷蔚佰,刘泰福,等主编.肿瘤放射治疗学[M].北京:医科大学中国协和医科大学联合出版社,1997:232-237.
  • 2Puchner M J, Giese A. Tamoxifen - resistant glioma - cell subpopulations are characterized by increased migration and proliferation [ J ]. Int J Cancer,2000,86 (4) :468 - 473.
  • 3Berd D, McLaughlin C J, Hart E, et al. Short course high dose tamoxifen with cytotoxic chemotherapy for metastatic melanoma [J]. Proc Am Soc Cli Oncol ,1991,10:291.
  • 4Couldwell WT, Weiss MH, DeGiorgio CM, et al. Clinical and radiographic response in a minority of patients with recurrent malignant gliomas treated with high -dose tamoxifen[ J]. Neurosurgery, 1993,32:485 - 490.
  • 5Burger PC, Dubois PJ, Schold Jr, et al. Computerized tomographic and pathologic studies of the untreated,quiescent,and recurrent glioblastoma muhiforme[J]. J Neurosurg, 1983,58(2): 159-169.
  • 6Markman M, Iseminger KA, Hatch KD. Tamoxifen in platinum-refractory ovarian cancer: a gynecologic oncology group ancillary report[J]. Gynecol Oncol,1996,62(1):4-6.
  • 7Couldwell WT, Weiss MH, DeGiorgio CM, et al. Clinical and radiographic response in a minority of patients with recurrent malignant gliomas treated with high-dose tamoxifen[J]. Neurosurgery, 1993;32(3):485-490.
  • 8Couldwell WT, Antel JP, Yong VW. Enhanced protein kinase C activity correlates with the growth rate of malignant gliomas:part II. Effects of glioma mitogens and modalators of PKC[J]. Neurosurgery ,1992,31(4):717-724.
  • 9Puchner M J, Giese A. Tamoxifen-resistant glioma-cell subpopulations are characterized by increased migration and proliferation[J].Int J Cancer,2000,86(4):468-473.
  • 10Brondan da Rocha A, Mans DRA, Bernard EA, et al. Tamoxifen inhibits particulate-associated protein kinase C activity, and sensitises cultured human glioblastoma cells not to etoposide but to γradiation and BCNU[J]. European Journal of Cancer, 1999,35(5):833-839.

共引文献1

同被引文献18

  • 1李富荣,齐晖,任莉莉,叶志中,洪小平,王新根.白细胞介素-18对系统性红斑狼疮患者外周血淋巴细胞早期凋亡信号Caspase-3表达的影响[J].免疫学杂志,2005,21(2):161-161. 被引量：2
  • 2程宏,祝瑾,贾筱琴.TRAIL与5-FU联合诱导白血病细胞凋亡的分子机理研究[J].扬州大学学报（农业与生命科学版）,2005,26(4):12-15. 被引量：3
  • 3Parkin D M, Bray F, Ferlay J, et al. Global cancer statistics, 2002 [J]. CA Cancer J Clin, 2005, 55(2): 74-108.
  • 4UNSCEAR report: sources and effects of ionizing radiation [S]. Manhattan: UN, 1993: 123.
  • 5IPCS. Environment Health Criteria 211. Health Effects of Interaction between Tobacco Use Exposure to Other Agents [M] Geneva: WHO, 1999: 57.
  • 6Darby S, Hill D, Anvinen A, et al. Radon in homes and lung cancer risk: collaborative analysis of individual data from 13 European case-control studies [J]. BMJ, 2005, 330(7485): 223.
  • 7Schmid K, Kuwert T, DreMer H. Radon in indoor spaces [J]. Dtseh Arztebl Int, 2010, 7(11): 181 -187.
  • 8Liu J, Liu J, Mao J, et al. caspase-3-medicated cyclic stretch induceded myoblast apoptosis via a Fas/FasL -inde- pendent singnaling pathway during myogenesis [J]. J Cell Biochem, 2009, 107(4) : 834-844.
  • 9Sasaki T, Kitamura T. Roles of FoxO1 and Sirtl in the central regulation of food intake [J]. Endocrine Journal, 2010, 57(11): 939-946.
  • 10Deng Xiang-qun, Cheng Jin-luo, Zhang Yun-ping, et al. Effects of caloric restriction on SIRT1 expression and apoptosis of islet beta cells in type 2 diabetic rats [J]. Acta Diabetologica, 2010, 47(1): 177-185.

引证文献2

二级引证文献8

扬州大学学报（农业与生命科学版）
2010年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部