文昌鸡促卵泡素受体基因多态性分析

Single nucleotide polymorphisms of follicle stimulating hormone receptor gene of Wenchang chicken

以促卵泡素受体(FSHR)基因作为影响鸡繁殖性状的候选基因,采用PCR-SSCP技术结合测序对编码FSHR胞外区部分的第1外显子(exon1)至第9外显子(exon9)共9个外显子区域进行单核苷酸多态性(SNPs)分析,寻找与鸡繁殖性能相关的遗传标记,为高繁殖力的标记辅助选择提供科学依据。结果表明:在exon2、exon4、exon6、exon8区域存在SNPs位点,分别在exon2片段中编码区5′端-49 bp处的C→T突变;exon4片段中编码区43 bp处的T→C突变,但未引起氨基酸的改变;exon6片段中编码区3′端+12 bp处的A→G突变;距exon8编码区3′端+38 bp处的G→T突变。经适合性检验,各基因的基因频率在群体内的分布均处于Hardy-Weinberg平衡状态(P>0.05)。FSHR是促卵泡素的特异性受体,这些位点的多态为进一步研究FSHR基因多态性对文昌鸡繁殖性能的遗传效应奠定了基础。

The objectives of the present study were to detect polymorphisms in the follicle stimulating hormone receptor （FSHR） gene, and to provide a scientific basis for marker assisted selection for high prolificacy in Wenchang chicken. In this study, the FSHR gene as the candidate gene of chicken＇s reproductive trait was analyzed on its exonl to exon9 regions which encode most of the extracellular domain by PCR-SSCP. The result showed that 4 SNPs sites were found at exon 2 region, 4 region, 6 region, 8 region respectively. They are C^-49→T^-49 mutation before the CDS＇ 5-flanking terminal of the exon2 region, T^43→C^43 mutation in the CDS of the exon 4 region, A^12→G^12 mutation after the CDS＇ 3-flanking terminal of the exon6 region, G^38→T^38 mutation after the CDS＇ 3-flanking terminal of the exon8 region. The fitness test indicated the gene frequency were all in Hardy-Weinberg balanced state （P〉0.05）. In conclusions, the polymorphisms of these sites lay a foundation for the further research on relationship between the polymorphisms of FSHR gene and the reproductive performance of Wenehang chicken.