2008年龙岩市手足口病实验室检测结果分析

Clinical laboratory testing of hand foot mouth disease in 2008,Longyan city

目的了解龙岩市2008年手足口病病原特点。方法用逆转录聚合酶链反应(RT-PCR)及组织细胞分离法,对614例手足口病患者的咽拭样本454份、粪便样本377份、疱疹液样本62份共893份进行CA16和EV71的检测。结果 614例中158例检出CA16或EV71,其中CA16 37例、EV71 121例,4例检出其他肠道病毒。病原监测发现2008年4月以前流行的是CA16,5月份CA16和EV71同时存在,6月份以后7个县(市、区)监测的病例均为EV71,全市7个县(市、区)均监测到EV71。结论本检测表明RT-PCR用于手足口病CA16和EV71的检测,可以快速、准确地扩增咽拭、粪便、疱疹液及病毒培养物中的病毒,鉴定病毒亚型,结果可靠,是一种快速、敏感、特异的检测方法。2008年引起龙岩市幼儿手足口病的病原,5月份以前流行病原为CA16,5月份后为EV71。

Objective To study the characteristics of hand foot mouth disease in 2008,Longyan city.Methods Cell culture and RT-PCR were used for testing the viral infection in 454 laryngeal swab,377 excrement and 62 herpes fluid specimens collected from 614 patients with hand foot mouth disease.Results One hundred and sixty-two cases of hand foot mouth disease（26.4%）were confirmed with the infection of viruses,which included CA16（37）,EV71（121）and other enteric viruses（4）.CA16 viral infection was prevalent before April.Viral infection of CA16 and EV71 were coexisted in May.EV71 was the only virus isolated from patients in June or later of 2008.EV71 virus was detected in the patients from all the 7 counties of Longyan city.Conclusion RT-PCR was a reliable clinical testing method for specimen of laryngeal swab,excrement and herpes fluid collected from patients with hand foot moth disease.The virus of CA16 and EV71 were respectively prevalent before and after May 2008 among the patients in Longyan city.