HPLC法定量分析30%克·多·福大豆种衣剂

Determination of Active Ingredients in 30% Carbo-furan-Carbendazim-Thiram SC for Seed Coating by HPLC

报道了３０％克·多·福大豆种衣剂的高效液相色谱分析方法，本方法采用异丙醇—水（每１０００ｍｌ水＋２ｍｌ氨水）为流动相，以２００ｍｍ×４０ｍｍ（ｉｄ）不锈钢柱，内装Ｎｏｖａ－ｐａｋＣ１８填充物（５μｍ），在２１０ｎｍ波长下进行测定。方法回收率克百威、多菌灵、福美双均在９８％～１０２％之间，标准偏差分别为０２４，０１３和０２０，变异系数分别为１６％，２６％和２０％。

A analytical method for determination of active ingredients in a sample of 30%carbofuran-carbendazim-thiram SC for soybean seed coating by HPLC is reported.The sample is dissolved in methanol,the carbofuran,carbendazim and thiram in the sample is separated and detenmined using 200mm×4 0mm(id)S.S tube packed with Nova-pak C 18 ,2-propanol-water as a mobile phase ,and UV detector at 210nm.The recoveries of the method for carbofuran,carbendazim,and thiram are all in a range of 98%～102%.SD and Cv of the method for carfuran,carbendazim,and thifram are 0.24,1,6%,0.13,2.6%,and 0.20,2.0%respefively.