子宫内膜癌中磷酸化哺乳动物雷帕霉素靶蛋白的表达及临床意义

Expression and significance of phosphorylation-mammalian target of rapamycin in endometrial carcinoma

目的 探讨磷酸化哺乳动物雷帕霉素靶蛋白（p-mTOR）在良恶性子宫内膜病变组织中的表达情况.方法 应用免疫组化SP法检测45例子宫内膜样腺癌、7例子宫内膜非典型增生、6例增殖期子宫内膜组织中p-mTOR的表达情况.结果 p-mTOR主要分布于细胞质,在增殖期子宫内膜中呈弱阳性表达或阴性表达,蛋白颗粒密度为2.36±0.76 在子宫内膜非典型增生和子宫内膜样腺癌中高表达,蛋白颗粒密度分别为6.21±1.19、15.82±2.64,三者间比较差异有统计学意义（F=11.37,P＜0.05）.在子宫内膜样腺癌组织中,不同组织学分级p-mTOR表达差异有统计学意义（F=8.27,P＜0.05）,但不同病理分期p-mTOR表达差异无统计学意义（P＞0.05）.结论 p-mTOR可能参与了子宫内膜癌的发生和发展过程.

Objective To investigate the expression of phosphorylation-mammalian target of rapamycin （p-mTOR） in endometrial carcinoma. Methods The expressions of p-mTOR protein in tissues from 45 cases of endometrial adenocarcinoma, 7 endometrial atypical hyperplasia, and 6 normal endometrium were detected by immunohistochemistry SP method. Results The expression of p-mTOR protein maily restricted to cytoplasm. Compared with normal endometrium, the expression of p-mTOR protein in the cases of endometrial atypical hyperplasia and endometrial adenocarcinoma was significantly up-regulation（2.36 ± 0.76vs 6.21 ± 1.19, 15.82 ± 2.64）（ F = 11.37, P 〈 0.05 ）. There was significant difference of p-mTOR protein in different histology class in endometrial adenocarcinoma （F = 8.27, P 〈 0.05）, but there was no significant difference of p-mTOR protein in different pathological grade in endometrial adenocarcinoma （P 〉0.05）.Conclusion The expression of p-mTOR protein may participate in the occurrence and development of endometrial carcinoma.