摘要
目的 观察七氟烷预处理对缺血/再灌（ischemia/reperfusion,I/R）损伤大鼠心肌磷酸化抑制蛋白（phosphorylation inhebitory kappaB,p-IκBα）的影响,从另一角度进一步探讨NF-κB在七氟烷预处理中的保护机制.方法 SD雄性大鼠56只,建立大鼠心肌I,R损伤在体模型,随机分为7组：假手术组（CON组）;单纯缺血组（I/R组）进行30 min心肌缺血后再灌注120min;七氟烷组（SEVO组）于吸入1.0 MAC七氟烷30 min,继之15 min药物排出后进行心肌I/R;SEVO 165＇组吸人七氟烷30min后停止吸人165 min;小白菊内酯（parthenolide,PTN）组于缺血前60 min腹腔内注射NF-κβ特异性抑制剂PTN 500 μg/kg;PTN＋SEVO组、SEVO＋PTN组分别于七氟烷预处理前15 min、预处理后即刻腹腔内注射PTN 500 μg/kg.分别于大鼠心肌I/R前、后或相应时间点取心肌标本,采用Western blotting法测定p-IκBa的蛋白表达.结果 缺血前即刻：SEVO组（22±3）和SEVO 165＇组（20±4）较CON组（15±3）p-IκBα蛋白表达上调（P〈0.05）;再灌注2 h后即刻：I/R组（44±6）和SEVO组（30±3）较CON组（15±4）p-IκBα蛋白表达均上调（P〈0.05）,而与I/R组相比,SEVO组p-IκBα蛋白上调幅度减小（P〈0.05）.结论 七氟烷预处理早期p-IκBα蛋白的表达上调,反馈性抑制了I/R后p-IκBα蛋白的表达,该作用可被PTN所阻断.从另一角度进一步论证了NF-κB参与七氟烷预处理的心肌保护机制.
Objective To observe the effect of sevoflurane preconditioning on the expression of p-IκBα in a rat model of myocardial ischemia-reperfusion （I/R）. Methods Eighty -four male SD rats after setting up the model of I/R, randomly were divided into seven groups： ①Control group; ② Simple-Ischemic group received 30 min I/R merely; ③ SEVO group inhaled 1.0 MAC sevoflurane for 30 min and 15 min wash-out followed by a 30 min I/R; ④ SEVO 165＇ group inhaled 1.0 MAC sevoflurane for 30 min and then stoped for 165 min; ⑤ PTN group received PTN 500 μg/kg（NF-κB inhibitor） intraperitoneally 60 min before I/R; ⑥ PTN±SEVO group and⑦SEVO＋PTN group received PTN 500 μg/kg 15 min before and after sevoflurane preconditioning. Myocardium sample of all groups were collected before and after the time of I/R or the corresponding point in time. p- IκBα was determined by Western Blotting. Results The expression of p- IκBαwas significantly up-regulated in SEVO group（22±3）and SEVO 165＇group（20±4） than that in Control group （15±3） before I/R（P〈0.05）; After perfusion the expression of p- IκBαin Simple-Ischemic group（44±6）and SEVO group （30±3） was significantly up-regulated than that in Control group（ 15±4,P〈0.05）;but the up-regulated adjustment of SEVO group was smaller than that in Simple-Ischemic group （P〈0.05）. Conclusion This study further confirmed NF-κB participates in the I/R injury and it may play an important role in the mechanism of sevoflurane-induced cardioprotection.
出处
《国际麻醉学与复苏杂志》
CAS
2010年第3期203-206,210,共5页
International Journal of Anesthesiology and Resuscitation
基金
国家自然科学基金（30872453）
江苏省自然科学基金（BK2008166）
江苏省高校自然科学基础研究面上项目（08KJD32005）
苏州市第十三批科技发展计划（社会发展及医药）项目（2008-11）
