摘要
目的定量检测人体抗-HBs对乙肝免疫的重要性。方法用时间分辨的方法(TRFIA)、酶联免疫吸附实验(ELISA)的方法以及胶体金免疫层析(GICA)的方法比对三者结果的符合率。结果时间分辨的方法和ELISA与胶体金免疫层析的方法阳性吻合率分别为98.6%和97.7%。结论 ELISA和胶体金方法只能测量乙肝抗-HBs阴性阳性结果,但对设备要求简单,回报结果迅捷,适用于大量标本的初筛;而时间分辨给出了抗-HBs的量化结果,更容易动态观察乙肝的病情发展以及疫苗注射情况。
Objective Quantitative detection of human anti-HBs on the importance of hepatitis B immunization. Methods Compared the resut time-resolved method （TRFIA） , enzyme-linked immunosorbent as- say （ELISA） method, and colloidal gold immunochromatography assay （GICA）. Results Time-resolved meth- od and ELISA and colloidal gold immunochromatographic method of positive consistent rate was 98. 6% and 97.7%. Conclusion ELISA and colloidal gold method can only measure of anti-HBs negative HBV positive results, but the device requires is a simple, quick return results for screening a large number of specimens;and time-resolved given the quantitative results of anti-HBs is easier dynamic observe the progression of the disease, as well as hepatitis B vaccination of.
出处
《中国实用医药》
2010年第18期51-52,共2页
China Practical Medicine
