小麦黑胚病种子带菌的光镜和扫描电镜观察

MICROSCOPY AND SCANNING ELECTRON MICROSCOPY OF BLACK EMBRYO OF WHEAT SEEDS AND THE SEED BORNE FUNGI

检测表明京双１６、聊８９－１、聊８９－２、聊９１－１、聊９１Ｂ１８－２７－１及鲁麦１２６个小麦品种（品系）的黑胚病病粒率为７．６％～４５．７％，病情指数为３．２％～１３．５％，以聊８９－１最轻，鲁麦１２最重。借助光学显微镜和扫描电子显微镜对６个品种（品系）的黑胚种子和健康胚种子进行了比较观察，证实了黑胚种子胚中链格孢菌（Ａｌｔｅｒｎａｒｉａ）的存在，此外，亦观察到了黑胚种子寄藏有类似于长蠕孢属（Ｈｅｌｍｉｎｔｈｏｓｐｏｒｉｕｍ）、枝孢属（Ｃｌａｄｏｓｐｏｒｉｕｍ）等真菌的菌体。

Six varieties(lines) of wheat Jing Shuang 16, Liao 89-1, Liao 89-2, Liao 91-1, Liao 91B18-27-1, and Lumai 12 were tested for black embryo disease by means of specimen examination with microscope and SEM. Their percentage of black end seeds was 76%-457%, (disease index) 32%-135%. Liao 89-1 and Lumai 12 were damaged resulting in weak and serious symptoms. Alternaria from embryo of black end seeds was identified under microscope while other seed borne fungi such as Helminthosporium and Cladosporium were further discovered by SEM