血清神经元特异性烯醇化酶与儿童失神癫癎关系的研究

Study of relationship between serum neuron-specific enolase and childhood absence epilepsy

目的:探讨血清神经元特异性烯醇化酶(NSE)在失神癫痫间儿童癫痫间发作后水平的变化及其与脑损伤的关系。方法:选择失神癫痫间儿童22例,根据过度换气是否能诱发失神癫痫间发作分为2个亚组:失神发作组10例,失神非发作组12例;正常对照组20例。应用酶联免疫法(ELISA)测定各组血清NSE水平。结果:失神发作组癫痫间发作后30min内,血清NSE水平显著高于失神非发作组及正常对照组,差异有统计学意义(P<0.01);而失神非发作组与正常对照组相比,差异无统计学意义(P>0.05)。结论:失神癫痫间发作后血清NSE水平明显升高,提示失神癫痫间发作后存在一定程度的脑神经元损伤,NSE可作为早期检测失神癫痫发作后神经元损伤的一个重要特异性生化指标。

Objective：To investigate the levels of neuron-specific enolase （NSE）in serum and to explore neuronal damage in absence epilepsy children after seizures. Methods：Twenty two children with absence epilepsy were enrolled, according to whether the hyperventilation induced absence seizures or not. The children were divided into two subgroups： 10 cases of absence seizures group/12 cases of non-seizure group, and twenty healthy children served as normal control group. The blood samples were collected within 30 minutes after seizure. An enzyme-linked immunosorbent assay（ELISA）was used to detect the serum NSE in all the children. Results： The serum levels of NSE in the absence seizures group were significantly higher than those in non-seizure group and normal control group（P 〈 0.01）;but there was no significant difference between non- seizures group and normal control group （P 〉 0.05）. Conclusion： The levels of serum NSE in children with absence epilepsy were markedly increased after seizures, suggesting that a certain degree of neuronal damage may result from absence seizures. Neuron-specific enolase may server as an important specificity of biochemical marker in the early detection of neuronal damage after absence seizures.