高效液相色谱法同时测定面粉中的过氧化苯甲酰和苯甲酸

Simultaneous determination of benzoyl peroxide and benzoic acid in flour by High performance liquid chromatography

目的:建立同时测定面粉中过氧化苯甲酰和苯甲酸的高效液相色谱法(HPLC)。方法:采用无水乙醇提取面粉中的过氧化苯甲酰和苯甲酸后,在Waters Symmetry C18柱上,以甲醇-0.05%磷酸水溶液(80:20)为流动相进行分离,HPLC-二极管阵列检测器(DAD)于235.5 nm测定。结果:过氧化苯甲酰和苯甲酸的最低检出限分别为0.14 mg/kg和0.20 mg/kg;过氧化苯甲酰的回收率和相对标准偏差分别为97.50%～101.60%和1.51%;苯甲酸的回收率和相对标准偏差分别为95.60%～104.20%和3.03%。结论:该方法具有准确、可靠、操作简便、检测限低等特点,适用于面粉中过氧化苯甲酰和苯甲酸的同时测定。

Objective：To simultaneously determine benzoyl peroxide and benzoic acid in flour with High performance liquid chromatography.Methods：The benzoyl peroxide and benzoic acid in flour were extracted with anhydrous alcohol.The HPLC separation was carried out with a Waters Symmetry C18 column using methanol-water contaning 0.05% phosphate acid（80：20） as mobile phase and detected by DAD at 235.5 nm.Results： The limit of detection（LOD） was 0.14 mg/kg and 0.20 mg/kg for benzoyl peroxide and benzoic acid,respectively.The recovery and RSD of benzoyl peroxide are 97.50%～101.60% and 1.51%,respectively;and which of benzoic acid are 95.60%～104.20% and 3.03%,respectively.Conclusion：This method is accurate and sensitive,easy to operate for simultaneously analyze the benzoyl peroxide and benzoic acid of flour.