OPA柱前衍生-高效液相色谱法测定氨基葡萄糖

Method for determine of glucosamine by HPLC system with o-phthaldialdehyde precolumn derivatization

目的:建立氨基葡萄糖含量测定的高效液相色谱-紫外检测器分析法。方法:样品经OPA柱前衍生处理,使用ZORBAX Eclipse XDB-C18(4.6×250 mm,5μm)色谱柱,流动相为甲醇+乙腈+水=15+15+70,流量1.0 ml/min,检测波长330 nm。结果:方法检出限为20 ng,回收率为98.3%～102.5%,线性范围0.02 mg/ml～1 mg/ml,相关系数为0.9993,标准偏差(RSD)为0.27%～2.98%。结论:本方法检测灵敏度高,线性范围宽,适用于氨基葡萄糖盐酸盐或硫酸盐的测定。

Objective：A HPLC-VWD metheod was established for the determination of glucosamine.Methods：Under the column ZORBAX Eclipse XDB-C18（4.6×250 mm,5 μm） and the mobile phase consisted of methanol-acetonitril-water（15-15-70） at a rate 1.0 ml/min,the derivative chemical which was derived by o-phthaldialdehyde precolumn were detected by HPLC-VWD,the detection wavelength was 330 nm.Results：Under such conditions,the peak areas was linear to the concentration of glucosamine in the range of 0.02 mg/ml～1.00 mg/ml with correlation coefficient r=0.9993.The detetion limit for glucosamine was 20 ng and RSD=0.27%～2.98% with the coverage of 98.3%～102.5%.Conclusion：The method had the high sensitivity,wide range for liner and it had been applied to the ditermination of glucosamine with satisfactory results.