异尖线虫Ⅲ期幼虫的理化耐受试验观察

Study on physicochemical characterization of third stage anisakis simplex larvae(L_3)

目的:了解异尖线虫Ⅲ期幼虫(L3)理化生物学特性,为防治异尖线虫病提供依据。方法:在实验室条件下,观察异尖线虫L3在不同的介质、抗蠕虫药、温度下的生存耐受情况。结果:异尖线虫L3对常用的调味品有较强的耐受力,高度白酒杀虫效果优于低度白酒;低浓度复方甲苯咪唑(6.25 g/L)杀虫效果优于高浓度(12.50 g/L、18.75 g/L)的,也优于其它药物;异尖线虫L3对低温有较强的耐受力,在-20℃、-10℃时最长分别可存活9 h、12 h,对高温非常敏感,50℃、60℃时分别在<11 s、<1 s即死。结论:异尖线虫L3对外界环境有较强耐受能力,海鱼上市前在-20℃冰冻24 h,烹饪时要温度高时间足够,人们养成良好的饮食习惯,可有效预防和控制人体异尖线虫病感染。

Objective：To observe the characteristics of biology and physicochemical of the third stage anisakis simplex larvae（L3） and to provide the basis of the anisakiasis prevention and control.Methods：The survival tolerance of the third stage anisakis simplex larvae（L3） in various medium,anthelmintic drug,temperature were observed in laboratory condition.Results：The third stage anisakis simplex larvae（L3） had a high endurance to stock condiment and the anisakicidal effects of the high purity wine was more effective than that of the low purity wine.The anisakicidal effects of 6.25 g/L mebendazole composite was more effective than that of 18.75 g/L and also more effective than that of another drugs.The third stage anisakis simplex larvae（L3） could be alive with length up to 9 h and 12 h in condition of-20℃,-10℃ and very sensitive to high temperature treatment.,barely less than 11s and 1s could be alive in 50℃ and 60℃.Conclusion：The third stage anisakis simplex larvae（L3） was shown to have a fairly good tolerance to the external environments.There time the marine fish freeze in condition of-20℃ beyond 24 h before their come into the market,So cooking with high temperature,long time combine with good dietary habit are useful to effective prevention and control in infect of anisakis.