内源及外源植物激素对陆地棉无絮突变体胚珠纤维初始发育诱导效应的研究(英文)

Effects of Endogenous and Exogenous Phytohormones on Fiber Initiation on Ovules from A Fuzzless lintless Mutant vs. Its Isogenic Wild type Line in Upland Cotton

采用陆地棉徐州１４２无絮突变体（ＦＬ）与其正常品种（ＷＴ）比较，研究了外源激素对离体胚珠纤维的诱导作用以及纤维初始发育过程中胚珠内源激素（ＩＡＡ，ＧＡ，ＡＢＡ和ｉＰＡ）含量的变化。结果表明，ＧＡ能够诱导离体的正常和突变体未受精和受精胚珠产生出纤维，开花前２ｄ～３ｄ的胚珠培养到天然开花日之后纤维方才突起。开花当天内源ＩＡＡ含量的急剧上升可能是胚珠表皮细胞迅速伸长的一个重要原因。开花前４ｄ到开花后４ｄ突变体胚珠内高水平的ｉＰＡ阻碍其纤维发生。

The effects of plant growth substances on fiber development in upland cotton were studied in this paper by contrasting the induction of cotton fiber in vitro and the fluctucation of the endogenous phytohormones IAA, GA 3, iPA, and ABA during fiber initation of a fuzzless lintless mutant (FL) with those of its wild type commercial cultivar Xuzhou 142(WT). Unfertilized and fertilized ovules of both WT and mutant can be initiated to produce fiber in BT with GA 3 added. Ovules aged 3 days and 2 days were cultured in vitro until the equivalent of 0 day when fiber development was initiated. This result is completely in accordance with the in vivo situation. The remarkably raised IAA level at anthesis may be responsible for the epidermal cell expansion. The increased levels of iPA in ovules from 4 days preanthesis to 4 days postathesis suppress fiber initiation of FL.