平阳霉素-活性炭纳米微粒对口腔鳞癌细胞系Tca8113和BcaCD885细胞体外的杀伤作用

Anticancer effects of Pingyangmycin-activated carbon nanoparticles against human oral squamous carcinoma Tca8113 and BcaCD885 cell lines in vitro

目的探讨平阳霉素-活性炭纳米微粒(PYM-CH-NP)对人舌鳞癌细胞系Tca8113和颊鳞癌细胞系Bca-CD885的药物敏感性。方法采用甲基噻唑基四唑(MTT)法检测不同剂量的PYM-CH-NP和平阳霉素(PYM)在7个时间点(1～7d)对Tca8113和BcaCD885的杀伤效应,计算各时间点2种剂型的半数抑癌率浓度值(IC50)、抑癌率和药物抗肿瘤作用强度的相对值(RAA),选择药物作用后第5天观察量效关系。结果 PYM-CH-NP和PYM对Tca8113和BcaCD885均有较强的抑癌作用,其抗癌效应与药物浓度和作用时间相关。结论改型后的PYM-CH-NP保留了PYM的抗癌活性,且具有缓释性,作用于肿瘤时可以维持周围有效的浓度和作用时间,有效地杀伤肿瘤细胞。

Objective The cytotoxic effects of a new formulation of Pingyangmycin-activated carbon nanoparticles（PYM-CH-NP） on two human oral squamous cell carcinoma Tca8113 and BcaCD885 cell lines were studied in vitro. Methods The inhibitory effects of PYM-CH-NP and Pingyangmycin（PYM） were evaluated by methyl thiazolyl tetrazolium（MTT） assay at 1-7 days. The 50% inhibition concentration values（IC50） and relative anti tumor activity（RAA） of PYM-CH-NP and PYM against Tca8113 and BcaCD885 with different drug concentration were evaluated. The time-dependent cytotoxic effects of PYM-CH-NP and PYM were during 1-5 days, so the doseeffect relationship was investigated at 5th day. Results Both PYM-CH-NP and PYM had high anticancer effects on Tca8113 and BcaCD885, and the cytotoxic effects were dose-dependent and time-dependent. Conclusion The activated carbon nanoparticles（CH-NP） may serve as a new drug delivery carrier of PYM. The new formulation PYM-CH-NP could slow down drug release, prolonged the drug concentration and its acting time, so more effective anticancer efficacy could be achieved.