慢性应激致抑郁模型大鼠红细胞促淋巴细胞增殖的变化

Red blood cell on lymphocyte proliferation in rats with chronic unpredictable mild stress

目的探讨慢性应激致抑郁模型大鼠各时程下红细胞促T、B淋巴细胞增殖的变化。方法将30只大鼠随机分为慢性应激抑郁模型组和正常对照组，进行相关行为学指标检测，采用四甲基偶氮唑蓝（MTT）比色法检测红细胞促淋巴细胞增殖反应的变化。结果造模7d后，与对照组相比，模型组大鼠体重增长减慢，大恼系数降低，差异有统计学意义（P〈0．05）。模型组大鼠水半及垂直运动得分、糖水消耗量、1％蔗糖偏爱百分比均降低，而纯水消耗量增加，差异有统计学意义（P〈0．05）。模型组血浆中皮质酮浓度在给予慢性应激14d达高峰[（349．89±13．01）ng／ml]，应激21d有下降的趋势，为（253．73±22．56）ng／ml，与同一时间点的对照组[分别为（218．50±16．85）、（209．83±9．64）ng／ml]比较，差异均有统计学意义（P〈0．05）。模型组大鼠与应激前相比，红细胞促T、B淋巴细胞增殖率降低，应激后第7、14、21天，红细胞促T淋巴细胞增殖率分别为1．40％＋0．09％、1．21％±0．25％、0．96％±0．13％，红细胞促B淋巴细胞增殖率分别为1．22％±0．06％、1．08％±0．10％、0．81％±0．21％，与对照组（分别为1．70％±0．15％、1．54％±0．24％）比较，差异均有统计学意义（P〈0．05）。结论慢性应激叮诱发大鼠较长时间的行为及活动习性的改变而导致抑郁，此过程中红细胞促淋巴细胞免疫功能受到影响。

Objective To study the changes of rat red blood cells on T, B lymphocytes proliferation in chronic unpredictable mild stress （CUMS）. Methods Thirty Wistar rats were averagely divided into control group and the CUMS group. The relevant behavioral performance and red blood cells on lymphocytes proliferation Changes by MTT colorimetric were observed. Results After fourteen days, the growth of weight and organic coefficient of brain decreased in model group （P〈0.05）, with a significantly reduced consumption and preference of sucrose solutions, and increased pure water consumption as compared with control group （P〈 0.05 ）. Plasma corticosterone levels peaked at seven days but on a declining trend after fourteen days. The rate of red blood cells on T, B lymphocyte proliferation in depression group was lower than non-stress in 7, 14, 21 days （P〈0.05）. Conclusion The repeated chronic mild stress stinmlation can induce long-time changes in acts and activities and decrease the rate of red blood cells on T, B lymphocytes proliferation, which can provide a new experimental method to observe the red blood cell imnmne function under the stress.