丛枝菌根真菌系统分类及群落研究技术进展

Systematic classification and community research techniques of arbuscular mycorrhizal fungi:A review

丛枝菌根真菌(AMF)是自然生态系统重要的组成部分,能与植物根系形成互惠共生体.传统的AMF分类主要依赖于对土壤无性孢子的形态鉴定,具有一定的局限性.近年来基于核酸分析的分子鉴定技术使AMF的分类更具科学性和准确性,补充和完善了基于孢子形态鉴定所建立的分类系统.AMF群落研究依赖于AMF的分类鉴定,主要包括孢子形态鉴定和分子生物学分析两类研究法.本文综述了AMF的分类系统和群落研究方法,着重介绍了近年来应用较多的AMF群落研究的分子生物学技术.作者认为,采取形态与分子相结合的办法将有助于推动AMF群落研究和AMF自然分类系统的建立和完善.

Arbuscular mycorrhizal fungi （AMF） are an important component of natural ecosystem,being able to form symbiont with plant roots. The traditional AMF classification is mainly based on the morphological identification of soil asexual spores,which has some limitations in the taxonomy of AMF. Advanced molecular techniques make the classification of AMF more accurate and scientific,and can improve the taxonomy of AMF established on the basis of morphological identification. The community research of AMF is mainly based on species classification,and has two kinds of investigation methods,i.e.,spores morphological identification and molecular analysis. This paper reviewed the research progress in the systematic classification and community research techniques of AMF,with the focus on the molecular techniques in community analysis of AMF. It was considered that using morphological and molecular methods together would redound to the accurate investigation of AMF community,and also,facilitate the improvement of AMF taxonomy.