增生性瘢痕水泡液β转化生长因子的变化及意义

TGF-β change in blister fluid of hypertrophic scar and its significance

目的 研究早期瘢痕组织β转化生长因子（transforming growth factor β,TGF-β）的变化,以探索TGF-β在增生性瘢痕形成中的作用,并利用瘢痕水泡液作为研究增生性瘢痕的新途径.方法 采用酶联免疫吸附法（ELISA）检测大面积深度烧伤患者愈合后瘢痕增生早期（≤90 d）的水泡液和血液TGF-β1的含量,动态监测瘢痕早期组织TGF-β1的变化,并与正常皮肤组织水泡液和血液对照.结果 大面积深度烧伤愈合后瘢痕形成早期患者血液和正常皮肤水泡液TGF-β1的含量与正常人血液比较无升高.均低于本试剂盒最低可检测阈值（15.6 pg/L,P〉0.01）;增生性瘢痕水泡液TGF-β1含量显著高于其血液和正常皮肤水泡液（P均〈0.01）,而且瘢痕增生早期（1～3个月）组织TGF-β1逐渐升高（P〈0.01）.结论 瘢痕形成早期组织TGF-β含量增高可能是瘢痕增生的重要因素;瘢痕水泡液可作为研究增生性瘢痕的新途径.

Objective To investigate of the tissue TGF-β changes at early stage of hypertrophic scar formation and the value of scar blisters in hypertrophic scar.Methods The TGF-β1 content in the blister fluid and the blood were quantified with ELISA,patients（n=15）with hypertrophy scar after depth burn were included,three time point（each n=5）on early stage（〈3 months）of hypertrophy scar formation were monitored.and normal skin blister fluid and the blood（n=5）was used as control.Results The serum TGF-β1 in the both hypertrophic scar patients and normal skin group was not elevated（P〉0.01）,the TGF-β1 in the blister of normal skin was also not elevated（P〉0.01）,but TGF-β1 level in the scarblisters hypertrophic scar was elevated significantly[〈60 d（158.5±69.8）pg/L,60-90 d,（181.1±40.1）pg/L,〉90 d,（534.4±125.9）pg/L,P〈0.01] and higher than the normal skin blister and the blood（P〈15.6 pg/L.P〈0.01）,the increased TGF-β1 1evel in the hypertrophic scar blisters were persisted for at least three months.the TGF-β1 level of scar blister on the 3th month of hypertrophic scar formation reached a peak [（534.4±125.9）pg/L,P〈0.01].Conclusions The data in this study indicates that TGF-β production at the early stage of hypertrophic scar formation is increased and may play an important role in scar formation;scar blisters is a valuable approach in hypertrophic scar study.