离体冻伤模型创面愈合过程中基质细胞衍生因子1促表皮干细胞迁移的研究

Promotion effect of stromal cell-derived factor 1 on the migration of epidermal stem cells in the healing process of frostbite-wound model ex vivo

目的 了解离体皮肤冻伤模型创面愈合过程中,基质细胞衍生因子1（SDF-1）对创缘表皮干细胞（ESC）的运动趋化作用.方法 体外构建三维皮肤等价物全层冻伤模型（创面呈孔形）,免疫组织化学染色观察冻伤后3、7 d创缘间质内SDF-1的表达情况.另将冻伤模型分为对照组（创面区添加PBS 50 μL/孔）、SDF-1组（创面区添加100 ng/mL SDF-1,50μL/孔）和AMD3100组[创面区用100 ng/mL AMD3100（50μL/孔）处理30 min后,添加SDF-1 50μL/孔],观察各组创缘中ESC的分布变化.结果 免疫组织化学染色显示,冻伤后3、7 d创缘间质内SDF-1的表达逐渐增加.与对照组及AMD3100组相比,SDF-1组创缘基底层整合素β1阳性细胞数量增多,且部分阳性细胞向上层表皮迁移聚集,创缘ESC数量更多,表皮细胞层数增加.结论 SDF-1促进ESC向创缘迁移聚集参与创面修复,这可能是ESC参与创面修复过程的机制之一.

Objective To study the promotion effect of stromal cell-derived factor 1 （SDF-1） on the migration of epidermal stem cells （ESC） in the healing process of frostbite-wound model ex vivo. Methods A three-dimensional model of full-thickness frostbite of skin was constructed （with slot-like wound） out of skin equivalent. The expression of SDF-1 in wound stroma was observed with immunohisto-chemistry staining on post injury days （PID） 3 and 7. The model frostbite wounds were divided into control group （treated with PBS 50 μL per wound） , SDF-1 group （treated with 100 ng/mL SDF-1, 50 μL per wound） , and AMD3100 group [treated with 100 ng/mL AMD3100 （50 μL per wound） for 30 minutes, and then SDF-1 50 μL was added per wound]. The redistribution of ESC around wound was observed. Results The expression of SDF-1 in wound stroma increased gradually on PID 3 and 7. Compared with those in control and AMD3100 groups, there were more ESC and epithelial cell layers, and more integrin β1-positive cells appeared at the basal layer of wound in SDF-1 group, and some of the positive cells migrated upward to epidermis. Conclusions SDF-1 contributes to wound repair through promoting ESC to migrate toward and gather around wound edge. This may be one of the mechanisms of ESC participating in wound repair.