2008年全国自身抗体检测质量控制总结

Summary of the 2008 national autoantibody testing quality control

目的 了解我国自身抗体检测的现状,进一步规范和提高我国自身抗体检测的水平.方法 以自愿报名的方法确定参与单位.质量控制项同包括抗核抗体（ANA）、抗双链DNA（dsDNA）抗体、抗可提取核抗原（ENA）抗体、抗线粒体抗体（AMA）/抗平滑肌抗体（ASMA）和抗环瓜氨酸肽（CCP）抗体5项,共15份标本.标本发放及结果分析采用双盲操作.结果 共102家医院或科室参加质量控制活动.ANA、抗dsDNA抗体、AMA、ASMA和抗CCP抗体的正确率分别为70%、88%、93%、85%和79%;抗RNP抗体、抗Sm抗体、抗SSA抗体、抗SSB抗体和抗Scl-70 正确率分别为88%、77%、92%、93%和87%.结论 我国自身抗体检测情况与前4次质鼍控制相比,参与质量控制的医院和检测的项目逐年增多,ANA、抗dsDNA抗体、抗ENA抗体和AMA检测水平降低,而ASMA和抗CCP抗体检测水平有所提高.

Objective To consecutively understand the national clinical testing status and to reassure the quality-control of autoantibody detection. Methods Letter or telephone notification were conducted to the participating hospitals or departments. Autoantibodies for quality control survey included anti-nuclear anti-body （ANA）, anti-double-stranded DNA （anti-dsDNA）, anti-extractable nuclear antigens （A-ENA）, anti-mitochondria antibody （AMA）/anti-smooth muscle antibody （ASMA）, and anti-citrulline antibody （anti-CCP）. Each had 3 control samples, and altogether 15 samples for testing. Sample distribution and data analysis were double-blinded. Results One hundred and two hospitals/departments participated in the national quality-control survey. The accurate rate for this survey was 70%, 88%, 93%, 85%, 79% respectively for ANA, anti-dsDNA, AMA, ASMA and anti-CCP. Anti-ENAs were further divided into anti-RNP, Sm, SSA, SSB and Scl-70 subgroups, and the accurate rate was 88%, 77%, 92%, 93% and 87% respectively. Conclu-sion Compared to the previous 4 national surveys, the accurate rates of ANA, anti-dsDNA, anti-ENAs, AMA in our country＇s autoantibody testing is improved, and the detection rate of ASMA and anti-CCP antibody is also increased. More hospitals and testing items can test these autoantibodies, which implies that autoantibody testing status has been improving in our country.