摘要
参考人、鸡、鼠的ESR 基因序列, 设计一对引物, 采用PCR 技术扩增出猪的ESR 基因一段DNA 片段。将该片段克隆到pGEMTEasy 质粒载体中, 重组质粒经PCR 鉴定和酶切分析确定克隆成功。经测序结果分析确定该片段为猪ESR 基因与其他动物第4 号外显子相对应的一段DNA 片段, 大小为332bp。
According to the data of the human,chicken and rat ESR sequence,a pair of primer is designed.The DNA fragment of the pig ESR gene was amplified successfully by PCR.The fragment was cloned into pGEM T Easy vector.Restriction enzyme analysis and PCR amplification of cloned fragment indicated the cloning successful.The result obtained from DNA sequencing show the DNA fragment is a fragment of exon 4 of the pig ESR gene and the length is 332bp.
出处
《湖北农业科学》
1999年第1期49-51,共3页
Hubei Agricultural Sciences
基金
"九五"国家重点科技攻关专题
湖北省农业科学院青年基金